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Anti-EIF4A2 Antibody (CAB3849)

SKU:
CAB3849
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • Immunology Antibodies 2 Anti-EIF4A2 Antibody CAB3849
  • Immunology Antibodies 2 Anti-EIF4A2 Antibody CAB3849
  • Immunology Antibodies 2 Anti-EIF4A2 Antibody CAB3849
  • Immunology Antibodies 2 Anti-EIF4A2 Antibody CAB3849
€139 - €419
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Description

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Antibody Name:Anti-EIF4A2 Antibody
Antibody SKU:CAB3849
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, U-251MG, Raji, HepG2, HT-1080, Mouse liver, Mouse brain, Mouse spleen, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSGG SADY NREH GGPE GMDP DGVI ESNW NEIV DNFD DMNL KESL LRGI YAYG FEKP SAIQ QRAI IPCI KGYD VIAQ AQSG TGKT ATFA ISIL QQLE IEFK ETQA LVLA PTRE LAQQ IQKV ILAL GDYM GATC HACI GGTN VRNE MQKL QAEA PHIV VGTP GRVF DMLN RRYL SPKW IKMF VLDE ADEM LSRG FKDQ IYEI FQKL NTSI QVVL LSAT MPTD VLEV TKKF MRDP IRIL VKKE ELTL EGIK QFYI NVER EEWK LDTL CDLY ET
Gene ID:1974
Uniprot:Q14240
Cellular Location:
Calculated MW:46kDa
Observed MW:46kDa
Synonyms:EIF4A2, BM-010, DDX2B, EIF4A, EIF4F, eIF-4A-II, eIF4A-II
Background:
UniProt Protein Function:EIF4A2: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Belongs to the DEAD box helicase family. eIF4A subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.6.4.13; Helicase; RNA-binding; Translation; Translation initiation

Chromosomal Location of Human Ortholog: 3q27.3

Cellular Component: cytosol; eukaryotic translation initiation factor 4F complex; perinuclear region of cytoplasm

Molecular Function:ATP-dependent RNA helicase activity; ATPase activity; helicase activity; protein binding

Biological Process: poly(A) tail shortening; RNA secondary structure unwinding; translational initiation

UniProt Code:Q14240
NCBI GenInfo Identifier:45645183
NCBI Gene ID:1974
NCBI Accession:Q14240.2
UniProt Secondary Accession:Q14240,Q53XJ6, Q96B90, Q96EA8, D3DNU9,
UniProt Related Accession:Q14240
Molecular Weight:
NCBI Full Name:Eukaryotic initiation factor 4A-II
NCBI Synonym Full Names:eukaryotic translation initiation factor 4A2
NCBI Official Symbol:EIF4A2  
NCBI Official Synonym Symbols:DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II  
NCBI Protein Information:eukaryotic initiation factor 4A-II
UniProt Protein Name:Eukaryotic initiation factor 4A-II
UniProt Synonym Protein Names:ATP-dependent RNA helicase eIF4A-2
Protein Family:Eukaryotic initiation factor
UniProt Gene Name:EIF4A2  
Anti-EIF4A2 Antibody (CAB3849)Western blot analysis of extracts of various cell lines, using EIF4A2 antibody (CAB3849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-EIF4A2 Antibody (CAB3849)Immunohistochemistry of paraffin-embedded rat brain using EIF4A2 antibody (CAB3849) at dilution of 1:100 (40x lens).
Anti-EIF4A2 Antibody (CAB3849)Immunohistochemistry of paraffin-embedded rat heart using EIF4A2 antibody (CAB3849) at dilution of 1:100 (40x lens).
Anti-EIF4A2 Antibody (CAB3849)Immunohistochemistry of paraffin-embedded mouse brain using EIF4A2 antibody (CAB3849) at dilution of 1:100 (40x lens).

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