The Fam221a Polyclonal Antibody (CAB20273) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human FAM221A and is suitable for use in Western blot and immunohistochemistry applications.FAM221A, also known as Family with Sequence Similarity 221 Member A, is a poorly characterized protein that has shown potential involvement in cell growth and differentiation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Fam221a Polyclonal Antibody
SKU:
CAB20273
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
D330028D13Rik, Fam221a
Positive Sample:
Mouse testis, Rat testis
Cellular Localization:
Cytoplasm.
Calculated MW:
33kDa
Observed MW:
33kDa
Orthologous to human FAM221A (family with sequence similarity 221 member A).
Purification Method
Affinity purification
Gene ID
231946
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Fam221a Rabbit pAb (CAB20273) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of A-549 cells using Fam221a Rabbit pAb (CAB20273) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
