Western blot analysis of extracts of Rosette leaf, using TIFY10A antibody (CAB21960) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The TIFY10A Polyclonal Antibody (CAB21960) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high sensitivity and specificity for detecting TIFY10A in human samples, making it a valuable asset for Western blot applications. By targeting the TIFY10A protein, this antibody enables precise detection and analysis in a variety of cell types, offering researchers insight into the role of TIFY10A in immunology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Arabidopsis thaliana samples.
Product Name:
TIFY10A Polyclonal Antibody
SKU:
CAB21960
Size:
20μL, 100μL
Reactivity:
Arabidopsis thaliana
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AtJAZ1, jasmonate-zim-domain protein 1, T29M8.5, T29M8_5, TIFY10A
Positive Sample:
Rosette leaf
Cellular Localization:
Nucleus, Plasma Membrane.
Calculated MW:
28kDa
Observed MW:
32kDa
JAZ1 is a nuclear-localized protein involved in jasmonate signaling. JAZ1 transcript levels rise in response to a jasmonate stimulus. JAZ1 can interact with the COI1 F-box subunit of an SCF E3 ubiquitin ligase in a yeast-two-hybrid assay only in the presence of jasmonate-isoleucine (JA-ILE) or coronatine. Application of jasmonate methyl ester to Arabidopsis roots reduces the levels of a JAZ1:GUS fusion protein, presumably by stimulating ubiquitin-proteasome-mediated degradation. The Jas domain appears to be important for JAZ1-COI1 interactions in the presence of coronatine. Two positive residues (R205 and R206) in the Jas domain shown to be important for coronatine -dependent COI1 binding are not required for binding AtMYC2.
Purification Method
Affinity purification
Gene ID
838501
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rosette leaf, using TIFY10A Rabbit pAb (CAB21960) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
