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GPA33 Rabbit Monoclonal Antibody (CAB3608)

SKU:
CAB3608
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
A33
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:GPA33 Rabbit Monoclonal Antibody
SKU:CAB3608
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 220-319 of human GPA33 (Q99795).
Sequence:QFCN ITVA VRSP SMNV ALYV GIAV GVVA ALII IGII IYCC CCRG KDDN TEDK EDAR PNRE AYEE PPEQ LREL SRER EEED DYRQ EEQR STGR ESPD HLDQ
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:A33; GPA33
Positive Sample:HCT 116,HT-29,A549,Mouse stomach,Rat large intestine
Conjugate:Unconjugated
Cellular Localization:Membrane, Single-pass type I membrane protein.
Calculated MW:36kDa
Observed MW:39kDa

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

Purification Method:Affinity purification
Gene ID:10223
Clone Number:ARC2060
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using GPA33 Rabbit mAb (CAB3608) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.

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