The GSDMD Monoclonal Antibody (CAB20728) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically designed for use in detecting GSDMD in human samples and is validated for use in Western blot applications. By binding to GSDMD, this antibody allows for the detection and analysis of GSDMD levels in various cell types, making it essential for studies in immunology and oncology research.GSDMD is a crucial player in the innate immune response, mediating the release of pro-inflammatory cytokines and inducing cell death in response to infection or cellular stress.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
GSDMD Monoclonal Antibody
SKU:
CAB20728
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC50993
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
79792
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from THP-1 cells, using GSDMD Rabbit mAb (CAB20728) at 1:1000 dilution. THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Western blot analysis of lysates from PC-3 cells using GSDMD (Full length+C terminal) Rabbit mAb (CAB20728) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
![Anti-GSDMD [R07-5C9] Monoclonal Antibody (AGMB03940)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/275229/677791/anti-gsdmd-r07-5c9-monoclonal-antibody-agmb03940__79721.1773033745.jpg?c=2 "Anti-GSDMD [R07-5C9] Monoclonal Antibody (AGMB03940)")
![Anti-GSDMD [R06-5C5] Monoclonal Antibody (AGMB01184)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/272473/692943/anti-gsdmd-r06-5c5-monoclonal-antibody-agmb01184__34231.1774507761.jpg?c=2 "Anti-GSDMD [R06-5C5] Monoclonal Antibody (AGMB01184)")
![Anti-Cleaved C-terminal GSDMD [R08-5D3] Monoclonal Antibody (AGMB03938)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/275227/677576/anti-cleaved-c-terminal-gsdmd-r08-5d3-monoclonal-antibody-agmb03938__33976.1773033034.jpg?c=2 "Anti-Cleaved C-terminal GSDMD [R08-5D3] Monoclonal Antibody (AGMB03938)")
![Anti-Cleaved C-terminal GSDMD [R09-7E6] Monoclonal Antibody (AGMB03939)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/275228/676098/anti-cleaved-c-terminal-gsdmd-r09-7e6-monoclonal-antibody-agmb03939__35476.1773028350.jpg?c=2 "Anti-Cleaved C-terminal GSDMD [R09-7E6] Monoclonal Antibody (AGMB03939)")
![Anti-Cleaved N-terminal GSDMD [R07-2C4] Monoclonal Antibody (AGMB03937)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/275226/676358/anti-cleaved-n-terminal-gsdmd-r07-2c4-monoclonal-antibody-agmb03937__72904.1773029184.jpg?c=2 "Anti-Cleaved N-terminal GSDMD [R07-2C4] Monoclonal Antibody (AGMB03937)")