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Anti-HIRA Antibody (CAB8892)

SKU:
CAB8892
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
DGCR1
TUP1
TUPLE1
Research Area:
Epigenetics and Nuclear Signaling
€139 - €419
Frequently bought together:

Description

Product Name:HIRA Rabbit mAb
Product Code:CAB8892
Size:20uL, 50uL, 100uL
Synonyms:DGCR1, TUP1, TUPLE1
Applications:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human HIRA
Applications:WB
Recommended Dilutions:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa, 293T
Immunogen:A synthesized peptide derived from human HIRA
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:7290
Uniprot:P54198
Calculated MW:112kDa
Observed MW:112KDa
UniProt Protein Function:HIRA: a histone chaperone. Necessary to mediate DNA-synthesis-independent nucleosome assembly. Interacts with UBN1, CABIN, and ASF1A in the cell nucleus to form the evolutionarily conserved HUCA histone chaperone complex that deposits the variant histone H3.3 into chromatin in a DNA-replication independent manner. Required for deposition of histone H3.3 at the transcription start sites of genes, where incorporation of histone H3.3 facilitates nucleosome destabilization and contributes to transcriptional activation. Histone H3.3 is also linked to gene silencing and is incorporated into regions of the genome thought to be transcriptionally inactive. While some incorporation of H3.3 into heterochromatin is facilitated by an additional histone chaperone complex containing ATRX and DAXX (ie. telomeric incorporation of H3.3), HIRA is required for incorporation of histone H3.3 and formation of senescence-associated heterochromatin foci (SAHF) during cellular senescence. HIRA is ubiquitously expressed during mouse embryonic development. In the adult mouse, HIRA is expressed at high levels in the kidney, skeletal muscle, and pancreas, but it is expressed at lower levels in the heart, lung, placenta, brain, and liver. A missing copy of the HIRA gene on human chromosome region 22q11.2 is a common characteristic of DiGeorge syndrome patients and insufficient production of the HIRA protein may disrupt normal embryonic development. Two isoforms of the human protein result from alternative splicing.
UniProt Protein Details:

Protein type:Transcription regulation; Cell development/differentiation; Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nuclear chromatin; nucleoplasm; nucleus; protein complex

Molecular Function:protein binding; transcription corepressor activity; transcription factor activity

Biological Process: anatomical structure morphogenesis; DNA replication-independent nucleosome assembly; regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]
UniProt Code:P54198
NCBI GenInfo Identifier:88984228
NCBI Gene ID:7290
NCBI Accession:P54198.2
UniProt Secondary Accession:P54198,Q05BU9, Q8IXN2,
UniProt Related Accession:P54198
Molecular Weight:89,299 Da
NCBI Full Name:Protein HIRA
NCBI Synonym Full Names:histone cell cycle regulator
NCBI Official Symbol:HIRA  
NCBI Official Synonym Symbols:TUP1; DGCR1; TUPLE1  
NCBI Protein Information:protein HIRA
UniProt Protein Name:Protein HIRA
UniProt Synonym Protein Names:TUP1-like enhancer of split protein 1
Protein Family:HIRA-interacting protein
UniProt Gene Name:HIRA  
UniProt Entry Name:HIRA_HUMAN

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