Anti-Human CD28 In Vivo Antibody

Product Name:	Anti-Human CD28 In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0325
Size:	1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:	CD28.2
Protein:	CD28
Product Type:	Monoclonal Antibody
Synonyms:	MGC138290, Tp44
Isotype:	IgG1 k
Reactivity:	Human
Applications:	Costim, FC, IHC FF, In Vivo, IP, PhenoCycler®
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	Costim, FC, IHC FF, In Vivo, IP, PhenoCycler®
Reactivity:	Human

Host Species:	Mouse
Specificity:	Clone CD28.2 recognizes an epitope on human CD28.
Antigen Distribution:	CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.
Concentration:	≥ 2.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.

Protein:	CD28
Ligand/Receptor:	CD80, CD86
Research Area:	Costimulatory Molecules, Immunology
Shipping:	2-8°C
PubMed:	CD28
NCBI Gene Bank ID:	940
UniProt:	CD28