Description
Anti-Human ICAM-1 In Vivo Antibody - Low Endotoxin
Introducing the Anti-Human ICAM-1 In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications.
This antibody targets the Intercellular Adhesion Molecule 1 (ICAM-1), a critical protein involved in the inflammatory response and immune cell adhesion, making it an excellent tool for research in immunology, inflammation, and related fields. With a mouse IgG1 isotype, it ensures high purity and low endotoxin levels (<0.1 EU/μg), ensuring optimal performance in ELISA, flow cytometry, immunohistochemistry, and other assays. Available in various sizes, it is formulated in phosphate-buffered saline for stability and efficacy. Enhance your research with this reliable and versatile antibody. ICAM-1 is a glycoprotein expressed on the surface of endothelial cells, immune cells, and some epithelial cells. It plays a significant role in the immune system by facilitating leukocyte endothelial transmigration, a key process in the inflammatory response, through interaction with integrins on leukocytes.
| Product Name: | Anti-Human ICAM-1 In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0134 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 15.2 |
| Protein: | Inter-Cellular Adhesion Molecule 1 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | BB2, CD54, P3.58 |
| Isotype: | Mouse IgG1 |
| Reactivity: | Human |
| Immunogen: | Human infant thymocytes and Sezary cells |
| Applications: | FC, IHC FFPE, IP, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FC, IHC FFPE, IP, WB |
| Reactivity: | Human |
| Host Species: | Mouse |
| Specificity: | Mouse Anti-Human Inter-Cellular Adhesion Molecule 1 (Clone 15.2) recognizes an epitope on Human ICAM-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| Antigen Distribution: | CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. |
| Immunogen: | Human infant thymocytes and Sezary cells |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.
| Datasheet: | View |
| Protein: | Inter-Cellular Adhesion Molecule 1 |
| Research Area: | Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cell |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
![Anti-Mouse ICAM-1 (CD54) [BE29G1] In Vivo Antibody - Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214503/569409/anti-mouse-icam-1-cd54-be29g1-in-vivo-antibody-low-endotoxin__35728.1770914078.jpg?c=2 "Anti-Mouse ICAM-1 (CD54) [BE29G1] In Vivo Antibody - Low Endotoxin")
![Anti-Mouse ICAM-1 (CD54) [BE29G1] In Vivo Antibody - Ultra Low Endotoxin](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/214502/569388/anti-mouse-icam-1-cd54-be29g1-in-vivo-antibody-ultra-low-endotoxin__83512.1770916022.jpg?c=2 "Anti-Mouse ICAM-1 (CD54) [BE29G1] In Vivo Antibody - Ultra Low Endotoxin")
