The ICAM1 Antibody (CAB20472) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced by Assay Genie, is generated using cutting-edge technology to ensure high specificity and sensitivity in detecting ICAM1 in various biological samples.ICAM1 is a cell surface glycoprotein that plays a crucial role in leukocyte migration and adhesion during immune responses. Dysregulation of ICAM1 expression is associated with various inflammatory diseases, making it a valuable target for research in immunology, inflammation, and related fields. The ICAM1 Monoclonal Antibody is ideal for experiments such as Western blotting, immunofluorescence, and flow cytometry to study ICAM1 expression and function in different cell types and tissues.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ICAM1 Antibody
SKU:
CAB20472
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD54, Ly-47, Icam-1, MALA-2, ICAM1
Positive Sample:
Rat lung
Cellular Localization:
Cell Surface, External Side Of Plasma Membrane, Extracellular Exosome, Extracellular Space, Immunological Synapse, Plasma Membrane.
Calculated MW:
59kDa
Observed MW:
85kDa
This gene encodes an integral membrane protein that binds leukocyte adhesion protein LFA-1. It participates in the innate immune response.
Purification Method
Affinity purification
Gene ID
15894
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung, using ICAM1 Rabbit pAb (CAB20472) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using ICAM1 Rabbit pAb (CAB20472) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using ICAM1 Rabbit pAb (CAB20472) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using ICAM1 Rabbit pAb (CAB20472) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
