The ICAM1 Antibody (CAB20472) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes an integral membrane protein that binds leukocyte adhesion protein LFA-1. It participates in the innate immune response. RRID Gene ID 15894 Swiss Prot Synonym CD54; Ly-47; Icam-1; MALA-2; ICAM1
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ICAM1 Antibody
SKU:
CAB20472
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD54, Ly-47, Icam-1, MALA-2, ICAM1
Positive Sample:
Rat lung
Cellular Localization:
Cell Surface, External Side Of Plasma Membrane, Extracellular Exosome, Extracellular Space, Immunological Synapse, Plasma Membrane.
Calculated MW:
59kDa
Observed MW:
85kDa
This gene encodes an integral membrane protein that binds leukocyte adhesion protein LFA-1. It participates in the innate immune response. RRID Gene ID 15894 Swiss Prot Synonym CD54; Ly-47; Icam-1; MALA-2; ICAM1
Purification Method:
Affinity purification
Gene ID:
15894
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung, using ICAM1 Rabbit pAb (CAB20472) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Mouse lung using ICAM1 Rabbit pAb (CAB20472) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using ICAM1 Rabbit pAb (CAB20472) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using ICAM1 Rabbit pAb (CAB20472) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
