The KDM3B Antibody (CAB2312) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable chromatin DNA binding activity; histone H3-methyl-lysine-9 demethylase activity; and transcription coregulator activity. Predicted to be involved in histone H3-K9 demethylation and regulation of transcription by RNA polymerase II. Located in nucleoplasm. Biomarker of acute lymphoblastic leukemia; breast cancer; colorectal cancer; and lung non-small cell carcinoma. RRID AB_2721243 Gene ID 51780 Swiss Prot Synonym 5qNCA; DIJOS; NET22; C5orf7; JMJD1B; KDM3B
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
KDM3B Antibody
SKU:
CAB2312
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
5qNCA, DIJOS, NET22, C5orf7, JMJD1B, KDM3B
Positive Sample:
HeLa
Cellular Localization:
Nucleus.
Calculated MW:
192kDa
Observed MW:
225kDa
Predicted to enable chromatin DNA binding activity; histone H3-methyl-lysine-9 demethylase activity; and transcription coregulator activity. Predicted to be involved in histone H3-K9 demethylation and regulation of transcription by RNA polymerase II. Located in nucleoplasm. Biomarker of acute lymphoblastic leukemia; breast cancer; colorectal cancer; and lung non-small cell carcinoma. RRID AB_2721243 Gene ID 51780 Swiss Prot Synonym 5qNCA; DIJOS; NET22; C5orf7; JMJD1B; KDM3B
Purification Method:
Affinity purification
Gene ID:
51780
RRID:
AB_2721243
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Hela cells, using KDM3B Rabbit pAb (CAB2312) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using KDM3B Rabbit pAb (CAB2312) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
