Western blot analysis of various lysates, using Connexin 43 antibody (CAB23120) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Connexin 43 Monoclonal Antibody (CAB23120) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal antibody technology, is highly specific to connexin-43 and is suitable for use in a variety of research applications, including immunofluorescence and immunohistochemistry.Connexin-43 is a key player in maintaining tissue homeostasis and regulating cell growth and proliferation. Dysregulation of connexin-43 has been implicated in various diseases, including cardiovascular disorders, neurodegenerative diseases, and cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Connexin 43 Monoclonal Antibody
SKU:
CAB23120
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC58819
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations.
Purification Method
Affinity purification
Gene ID
2697
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from U-87 MG cells using Connexin 43 Rabbit mAb (CAB23120) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5 s.
Western blot analysis of lysates from Mouse brain using Connexin 43 Rabbit mAb (CAB23120) at 1:5000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5 s.
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