The MED4 Monoclonal Antibody (CAB0623) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the MED4 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it a versatile option for studies in molecular biology and cell signaling research.MED4 plays a crucial role in regulating gene expression, cell growth, and proliferation, making it an important target for understanding diseases such as cancer and neurological disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MED4 Monoclonal Antibody
SKU:
CAB0623
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2528
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ARC36, VDRIP, DRIP36, TRAP36, HSPC126, MED4
Positive Sample:
TE-1, Jurkat, U-87MG, Mouse lung, Rat liver
Cellular Localization:
Nucleus.
Calculated MW:
30kDa
Observed MW:
36kDa
This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
29079
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MED4 Rabbit mAb (CAB0623) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using MED4 Rabbit mAb (CAB0623) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human breast tissue using MED4 Rabbit mAb (CAB0623) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using MED4 Rabbit mAb (CAB0623) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using MED4 Rabbit mAb (CAB0623) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
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