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Anti-MEKK3 Antibody CAB3695
Key Features

MEKK3 Rabbit Monoclonal Antibody (CAB3695)

Datasheet MSDS
SKU CAB3695
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse
Host Species Rabbit
Synonyms MEKK3, MAPKKK3
Research Use Cell Biology
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

MEKK3 Monoclonal Antibody (CAB3695)

The MEKK3 Monoclonal Antibody (CAB3695) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has been extensively validated for use in Western blot and immunofluorescence applications, demonstrating high specificity and sensitivity in detecting MEKK3 in human samples.MEKK3, also known as mitogen-activated protein kinase kinase kinase 3, is involved in multiple cellular processes, including inflammation, stress response, and development.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:MEKK3 Monoclonal Antibody
SKU:CAB3695
Size:20μL, 100μL
Reactivity:Human, Mouse
Clone Number:ARC2074
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MDEQ EALN SIMN DLVA LQMN RRHR MPGY ETMK NKDT GHSN RQSD VRIK FEHN GERR IIAF SRPV KYED VEHK VTTV FGQP LDLH YMNN ELSI LLKN QDDL
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MEKK3, MAPKKK3
Positive Sample:HeLa, NIH/3T3, Jurkat, SH-SY5Y, Mouse spleen
Cellular Localization:Cytoplasm, Cytosol.
Calculated MW:71kDa
Observed MW:78kDa

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification MethodAffinity purification
Gene ID4215
RRIDAB_2863120
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using MEKK3 Rabbit mAb (CAB3695) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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