null

Anti-MMP7 Antibody (CAB20701)

SKU:
CAB20701
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
  • Immunohistochemistry of paraffin-embedded human prostate cancer using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human breast cancer using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human brain using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
  • Western blot analysis of extracts of various cell lines, using MMP7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using MMP7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
€179 - €469
Frequently bought together:

Description

Antibody Name:Anti-MMP7 Antibody (CAB20701)
Antibody SKU:CAB20701
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human MMP7.
Application:Western blotting, Immunohistochemistry
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, 293T, A-549, BxPC-3, Mouse small intestine, Rat uterus
Immunogen:Recombinant protein of human MMP7.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Secreted, extracellular matrix, extracellular space
Calculated MW:29kDa
Observed MW:20-22KDa/28KDa
Synonyms:MMP7, MMP-7, MPSL1, PUMP-1
Background:This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal hemopexin domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes on chromosome 11. This gene exhibits elevated expression levels in multiple human cancers.
Immunohistochemistry of paraffin-embedded human prostate cancer using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human prostate cancer using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human breast cancer using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human brain using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human brain using MMP7 Rabbit mAb at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Western blot analysis of extracts of various cell lines, using MMP7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.Western blot analysis of extracts of various cell lines, using MMP7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using MMP7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using MMP7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Related Products