Description
Anti-Mouse CD25 In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Mouse CD25 In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody crafted for in vivo applications. This antibody is designed to target the CD25 protein, a critical component in the regulation and activation of the immune response, making it an essential tool for immunological research and related studies.
Characterized by its mouse IgG1 kappa isotype, this antibody ensures superior purity and ultra-low endotoxin levels (<0.01 EU/μg), optimizing it for applications such as ELISA, flow cytometry, immunohistochemistry, and more. Available in multiple sizes, it is formulated in phosphate-buffered saline, enhancing both stability and efficacy. Elevate your research endeavors with this dependable and versatile antibody. CD25, also known as the interleukin-2 receptor alpha chain, is prominently expressed on the surface of activated T-cells, regulatory T-cells, and a subset of B-cells. It plays a pivotal role in the immune system by facilitating the growth, proliferation, and differentiation of T-cells in response to immune signaling. The engagement of CD25 with its ligand, interleukin-2 (IL-2), is crucial for the modulation of immune responses and maintenance of immune homeostasis.
| Product Name: | Anti-Mouse CD25 In Vivo Antibody - Ultra Low Endotoxin |
| Product Code: | IVMB0149 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | PC61 |
| Protein: | CD25 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | IL2RA, IDDM10, CD25, IL2R, TCGFR, P55, Tac, IL-2 Rα |
| Isotype: | Rat IgG1 λ |
| Reactivity: | Mouse |
| Immunogen: | IL-2-dependent cytolytic mouse T-cell clone B6.1 |
| Applications: | B, Depletion, FA, FC, IHC FF, IP, PhenoCycler®, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CODEX®, Depletion, FA, FC, IHC FF, IP, WB |
| Recommended Usage: | FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 1 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this PC61 antibody for use in western blotting is 1-10 µg/ml. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone PC61 recognizes an epitope on mouse CD25. |
| Antigen Distribution: | CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells. |
| Immunogen: | IL-2-dependent cytolytic mouse T-cell clone B6.1 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | <0.5 EU/mg as determined by the LAL method |
| Purity: | ≥98% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.
| Datasheet: | View |
| Protein: | CD25 |
| Ligand/Receptor: | IL-2 |
| Research Area: | Immunology |
