Anti-Mouse CD3 (17A2) In Vivo Antibody

Product Name:	Anti-Mouse CD3 (17A2) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0321
Size:	0.5 mg, 1 mg, 5 mg
Clone:	17A2
Protein:	CD3
Product Type:	Monoclonal Antibody
Synonyms:	T-cell Receptor Complex, CD3ε
Isotype:	IgG2b k
Reactivity:	Mouse
Applications:	Costim, FC, IHC FF, In Vivo, PhenoCycler®
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	Costim, FC, IHC FF, In Vivo, PhenoCycler®
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone 17A2 recognizes an epitope on mouse CD3.
Antigen Distribution:	CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Immunogen:	gamma/δ TCR-positive T-T hybridoma D1
Concentration:	≥1.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	≥95% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, gamma and ζ chains, in addition to the TCR α/β or gamma/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Protein:	CD3
Function:	Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor:	Peptide antigen/MHC-complex
Shipping:	2-8°C
PubMed:	CD3
NCBI Gene Bank ID:	12502
UniProt:	CD3