Anti-Human CD8 In Vivo Antibody - Ultra Low Endotoxin

Product Name:	Anti-Human CD8 In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0125
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	UCHT-4
Protein:	CD8
Product Type:	Monoclonal Antibody
Isotype:	Mouse IgG2a
Reactivity:	Human
Immunogen:	Thymocytes and Sézary T cells
Applications:	FA, FC, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0124	Anti-Human CD8 In Vivo Antibody - Low Endotoxin	Low
IVMB0125	Anti-Human CD8 In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	FA, FC, WB
Recommended Usage:	FC The suggested concentration for this UCHT-4 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 10⁶ in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this UCHT-4 antibody for use in western blotting is 1-10 µg/ml when used in conjunction with compatible secondary reagents, under either reducing or non-reducing conditions. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Reactivity:	Human

Host Species:	Mouse
Specificity:	Clone UCHT-4 recognizes human CD8.
Antigen Distribution:	CD8 is expressed on blood lymphocytes, a subset of NK cells, and thymocytes. Persons with HIV exhibit increased levels of CD8+ lymphocytes.
Immunogen:	Thymocytes and Sézary T cells
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.

Technical Datasheet:	View
Protein:	CD8
Ligand/Receptor:	MHC Class I molecules
Research Area:	Immunology

Mouse IgG2a Isotype Control
Clone	C1.18.4
Isotype	Mouse IgG2a kappa
Endotoxin Level	Ultra Low Endotoxin

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