Anti-Human CD11c In Vivo Antibody

Product Name:	Anti-Human CD11c In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0110
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	3.9
Protein:	CD11c
Product Type:	Monoclonal Antibody
Synonyms:	Integrin αX subunit, CR4, p150, ITGAX
Isotype:	Mouse IgG1 κ
Reactivity:	Human
Immunogen:	Rheumatoid synovial fluid cells and fibronectin purified human monocytes
Applications:	B, CyTOF®, FA, FC, IHC FF, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0109	Anti-Human CD11c In Vivo Antibody - Low Endotoxin	Low
IVMB0110	Anti-Human CD11c In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	B, CyTOF®, FA, FC, IHC FF, WB
Recommended Usage:	FC The suggested concentration for clone 3.9 antibody for staining cells in flow cytometry is ≤ 2.0 µg per 10⁶ in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application.> WB The suggested concentration for this 3.9 antibody for use in western blotting is 1-10 µg/ml. A suggested positive control for Western blotting is a Mouse Skeletal Muscle Tissue Lysate.
Reactivity:	Human

Host Species:	Mouse
Specificity:	Clone 3.9 recognizes the α-chain (CD11c) of the CD11c/CD18 complex. It is specific for the I domain of CD11c. Clone 3.9 binds the activated form of CD11c and partially blocks the binding of CD11c with ICAM-4.
Antigen Distribution:	CD11c is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells.
Immunogen:	Rheumatoid synovial fluid cells and fibronectin purified human monocytes
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or β may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the β2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/β heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the β2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.

Technical Datasheet:	View
Protein:	CD11c
Function:	Adhesion, CTL killing
Ligand/Receptor:	CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
Research Area:	Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers

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