Anti-Human CD2 In Vivo Antibody - Ultra Low Endotoxin
Introducing the Anti-Human CD2 In Vivo Antibody - Ultra Low Endotoxin from Assay Genie, a highly specific monoclonal antibody engineered for in vivo experiments. This antibody specifically targets the CD2 protein, which is pivotal in T-cell activation and adhesion, making it an excellent choice for immunological research and related studies. Boasting a rat IgG2b isotype, it ensures exceptional purity and ultra-low endotoxin levels (<0.01 EU/mg), making it ideal for applications such as ELISA, flow cytometry, and immunohistochemistry.
Available in multiple sizes, this antibody is meticulously formulated in phosphate-buffered saline to guarantee stability and efficacy. Elevate your research with this dependable and versatile antibody. CD2 is a surface glycoprotein primarily found on T-cells and natural killer (NK) cells. It crucially contributes to the immune system by facilitating T-cell adhesion and signal transduction, thus playing an essential role in the body's immune response.
Product Name:
Anti-Human CD2 In Vivo Antibody - Ultra Low Endotoxin
Product Code:
IVMB0264
Size:
1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:
G11
Protein:
CD2
Product Type:
Monoclonal Antibody
Isotype:
IgG2a
Reactivity:
Human
Applications:
Costim, FC, IHC, In Vivo, WB
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Purity:
≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:
Costim, FC, IHC, In Vivo, WB
Reactivity:
Human
Host Species:
Mouse
Specificity:
Clone G11 recognizes an epitope of human CD2.
Antigen Distribution:
CD2 is present on normal peripheral blood lymphocytes, thymocytes, mature circulating T-cells and a subset of NK cells.
Concentration:
≥ 5.0 mg/ml
Endotoxin Level:
<0.5 EU/mg as determined by the LAL method
Formulation:
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:
≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
CD2 is a 45-59 kD type I transmembrane glycoprotein belonging to the Ig superfamily. CD2 is the receptor for LFA-3/CD58. CD2 serves as an adhesion receptor that binds to CD58; generating the activation of CD2-positive T cells and NK cells and in the regulation of their cytolytic activities. CD2 is a specific marker for T cells and NK cells. Hence, it can be used in immunohistochemistry to identify the occurrence of these cells in tissue sections. The vast majority of T cell lymphomas and leukemias express CD2 which makes it possible to distinguish these conditions from B-cell lymphomas.
Protein:
CD2
Ligand/Receptor:
CD58 (LFA-3), CD48, CD59, CD15
Research Area:
Costimulatory Molecules, Immunology
Pathogen Testing:
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
