Anti-Human CD3 [UCHT-1] In Vivo Antibody

Product Name:	Anti-Human CD3 [UCHT-1] In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0117
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	UCHT-1
Protein:	CD3
Product Type:	Monoclonal Antibody
Synonyms:	T3, CD3-epsilon
Isotype:	Mouse IgG1 κ
Reactivity:	Human
Immunogen:	This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunized against human lymphocytes, with UCHT1 being one of the first successful fusions.
Applications:	Act, CyTOF®, FC, IF Staining, IHC FF, IP, PhenoCycler®, WB\nWorkshops III 471
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Applications:	Act, CODEX®, CyTOF®, FC, IF Staining, IHC FF, IP, WB
Recommended Usage:	FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 µg per 10⁶ in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB
Reactivity:	Human

Host Species:	Mouse
Specificity:	Clone UCHT-1 binds to an acidic region of CD3-epsilon, occluding this region from direct interaction with the T cell receptor. This antibody is considered a pan T-cell marker. Furthermore, this antibody can be used for the detection of T cell populations in peripheral blood, lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia’s. It reacts with the majority of peripheral blood T lymphocytes, a significant proportion of thymocytes, the majority of T cell chronic lymphocytic leukemia cells and approximately 70% of acute lymphoblastic leukaemia’s of T cell origin.
Antigen Distribution:	CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Immunogen:	This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunized against human lymphocytes, with UCHT1 being one of the first successful fusions.
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Technical Datasheet:	View
Protein:	CD3
Function:	Antigen recognition, signal transduction, T cell activation
Ligand/Receptor:	Peptide antigen bound to MHC
Research Area:	Immunology

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