Description
Anti-Mouse CD366 (Tim-3) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD366 (TIM-3) In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody designed for in vivo applications. This antibody targets the CD366 protein, also known as T cell immunoglobulin and mucin-domain containing-3 (TIM-3), which plays a crucial role in regulating immune responses.
Ideal for research in immunology, this antibody aids investigations in areas like cancer, autoimmune disorders, and infectious diseases. With a rat IgG2a isotype, it ensures high purity and low endotoxin levels (<1.0 EU/mg), making it suitable for various assays such as ELISA, flow cytometry, and immunohistochemistry. Available in multiple sizes, it is formulated in phosphate-buffered saline to ensure stability and efficacy. Enhance your research with this reliable and versatile antibody. CD366 (TIM-3) is a protein expressed on the surface of certain immune cells, including T cells, dendritic cells, and other myeloid cells. It is involved in the inhibition of Th1-mediated immunity and plays a role in immune tolerance and exhaustion, particularly in the context of chronic infections and cancer. By targeting CD366, this antibody supports the exploration of immune modulation and the development of novel therapeutic strategies.
| Product Name: | Anti-Mouse CD366 (Tim-3) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0097 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | RMT3-23 |
| Protein: | Tim-3 |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Havcr2 |
| Isotype: | Rat IgG2a κ |
| Reactivity: | Mouse |
| Immunogen: | This antibody was produced using recombinant mouse TIM-3 |
| Applications: | B, FC, IF Staining, IHC, IHC FF, PhenoCycler® |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | B, CODEX®, FC, IF Staining, IHC, IHC FF |
| Recommended Usage: | FC The suggested concentration for CD366 antibody (clone RMT3-23) for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone RMT2-23 recognizes an epitope on mouse Tim-3. |
| Antigen Distribution: | Tim-3 is expressed on activated Th1 and Tc1 lymphocytes, CD11b+ macrophages, Tregs, NK cells, and mast cells. |
| Immunogen: | This antibody was produced using recombinant mouse TIM-3 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Tim-3 antibody, clone RMT3-23, recognizes T cell immunoglobulin and mucin domain-containing protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim-3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucin-like domain and a cytoplasmic tyrosine phosphorylation motif. Tim-3 is expressed by IFNg-producing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells1, regulatory T cells (Tregs)2, myeloid cells3, NK cells4, and mast cells5. Tim-3 is an inhibitory molecule that limits Th1-mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)1, inflammatory bowel disease (IBD)6, and type I diabetes7. Tim-3 is also proposed to induce immunological tolerance7, promote Th1 apoptosis8, and regulate macrophage activation1. In addition, Tim-3 is upregulated on tumor-infiltrating lymphocytes, and coblockade of Tim-3 and immune checkpoint inhibitors, such as PD-1, is currently being investigated in clinical trials for the treatment of cancer9.
| Datasheet: | View |
| Protein: | Tim-3 |
| Function: | May play a role in the development of immune responses and the development of Th1-mediated responses |
| Ligand/Receptor: | Putative ligand on resting CD4+ lymphocytes |
| Research Area: | Immunology, Inhibitory Molecules |
![Anti-Mouse CD366/Tim-3 Antibody [RMT3-23] -Low Endotoxin Purified (AGEL2360)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/270440/692840/anti-mouse-cd366tim-3-antibody-rmt3-23-low-endotoxin-purified-agel2360__80823.1774507412.jpg?c=2 "Anti-Mouse CD366/Tim-3 Antibody [RMT3-23] -Low Endotoxin Purified (AGEL2360)")
