Description
Anti-Mouse CD8a (Ly 2.2) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse CD8a [Ly-2.2] In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody meticulously designed for in vivo applications. This antibody targets the CD8a protein, an essential molecule in the immune response, making it an indispensable tool for research in immunology and associated research fields. With a rat IgG2a kappa isotype, this antibody ensures high purity and low endotoxin levels (<1.0 EU/mg), making it ideal for applications such as ELISA, flow cytometry, immunohistochemistry, and other pivotal assays. It is available in various sizes and is formulated in phosphate-buffered saline to ensure stability and efficacy.
Elevate your research capabilities with this reliable and versatile antibody. CD8a, also known as Ly-2.2, is a glycoprotein found on the surface of immune cells such as cytotoxic T lymphocytes (CTLs). It plays a critical role in the immune system by aiding the activation of T-cells through interaction with major histocompatibility complex (MHC) class I molecules. Enhance your immunological research with the precision and reliability of the Anti-Mouse CD8a [Ly-2.2] In Vivo Antibody from Assay Genie.
| Product Name: | Anti-Mouse CD8a (Ly 2.2) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0070 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | 2.43 |
| Protein: | CD8a |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Ly 2.2, Ly-2, Ly-35, Ly-B, Lyt-2, Lyt2 |
| Isotype: | Rat IgG2b |
| Reactivity: | Mouse |
| Immunogen: | Mouse CTL clone L3 |
| Applications: | Depletion, FA, FC, ICC, IF Staining, IHC FFPE, IP |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | Depletion, FA, FC, ICC, IF Staining, IHC FFPE, IP |
| Recommended Usage: | FC The suggested concentration for this 2.43 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 106 in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Rat |
| Specificity: | Clone 2.43 recognizes an epitope on mouse CD8a. |
| Antigen Distribution: | CD8a is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic-cells. |
| Immunogen: | Mouse CTL clone L3 |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.
| Technical Datasheet: | View |
| Protein: | CD8a |
| Function: | Depleting activity when used in vivo. |
| Ligand/Receptor: | MHC class I molecule |
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| Turley et al. | Intratumoral delivery of the chitin-derived C100 adjuvant promotes robust STING, IFNAR, and CD8+ T cell-dependent anti-tumor immunity | Cell Reports Medicine 2024 | PubMed ID: 38729159 |
| Joanna L. Turley et al. | Intratumoral delivery of the chitin-derived C100 adjuvant promotes robust STING, IFNAR, and CD8+ T cell-dependent anti-tumor immunit | Cell Reports Medicine 2024 | PubMed ID: 38729159 |
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