Anti-Mouse Ly-6G In Vivo Antibody

Product Name:	Anti-Mouse Ly-6G In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0025
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	1A8
Protein:	Ly-6G
Product Type:	Monoclonal Antibody
Synonyms:	Lymphocyte antigen 6 complex, locus G
Isotype:	Rat IgG2a κ
Reactivity:	Mouse
Immunogen:	Mouse Ly-6G transfected EL-4J cell line
Applications:	CyTOF®, Depletion, FC, IHC FF, PhenoCycler®, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0025	Anti-Mouse Ly-6G In Vivo Antibody - Low Endotoxin	Low
IVMB0026	Anti-Mouse Ly-6G In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	CODEX®, CyTOF®, Depletion, FC, IHC FF, WB
Recommended Usage:	FC The suggested concentration for this clone 1a8 antibody for staining cells in flow cytometry is ≤ 0.25 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone 1A8 recognizes an epitope on mouse Ly6G. Clone 1A8 does not cross react with Ly6C.
Antigen Distribution:	Ly6G is expressed by neutrophils.
Immunogen:	Mouse Ly-6G transfected EL-4J cell line
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Ly6G antibody, clone 1A8, recognizes lymphocyte antigen 6 complex locus G6D (Ly6G; also called Gr-1), a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein1. Ly6G belongs to the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) superfamily, characterized by a Ly6/uPAR (LU) domain-containing a three-fingered structural motif stabilized by disulfide bonds2. Ly6G is expressed by murine neutrophils regardless of location and activation1,4,5. Eosinophils may also express low levels of Ly6G5. There is no human ortholog for Ly6G; however, a structurally related L76/uPAR protein, CD177 (also known as HNA-2a, NB1, or PRV-1) is expressed in human neutrophils and is implicated in neutropenia6. Although the exact function and ligand of Ly6G remain unknown, Ly6G ligation may impair neutrophil migration to sites of inflammation via a β2-integrin-dependent mechanism7.

Technical Datasheet:	View
Protein:	Ly-6G
Research Area:	Immunology, Innate Immunity

Rat IgG2a Isotype Control
Clone	1-1
Isotype	Rat IgG2a
Endotoxin Level	Low Endotoxin

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