Anti-Mouse Ly-6C [HK1.4] In Vivo Antibody

Product Name:	Anti-Mouse Ly-6C [HK1.4] In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0177
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	HK1.4
Protein:	Ly-6C
Product Type:	Monoclonal Antibody
Synonyms:	Lymphocyte antigen 6 complex, locus C
Isotype:	Rat IgG2c
Reactivity:	Mouse
Immunogen:	L3 cloned CTL cells
Applications:	CyTOF®, IHC FF, IP, PhenoCycler®, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 20 mM Borate, 250 mM Sodium Chloride (NaCl), pH 8.3-8.7 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Applications:	CODEX®, CyTOF®, IHC FF, IP, WB
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Rat Anti-Mouse Ly-6C (Clone HK1.4) recognizes an epitope on Mouse Ly-6C. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:	The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5
Immunogen:	L3 cloned CTL cells
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 20 mM Borate, 250 mM Sodium Chloride (NaCl), pH 8.3-8.7 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Technical Datasheet:	View
Protein:	Ly-6C
Research Area:	Immunology

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