Anti-Mouse CD8a (Ly 2.2) In Vivo Antibody

Product Name:	Anti-Mouse CD8a (Ly 2.2) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0070
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	2.43
Protein:	CD8a
Product Type:	Monoclonal Antibody
Synonyms:	Ly 2.2, Ly-2, Ly-35, Ly-B, Lyt-2, Lyt2
Isotype:	Rat IgG2b
Reactivity:	Mouse
Immunogen:	Mouse CTL clone L3
Applications:	Depletion, FA, FC, ICC, IF Staining, IHC FFPE, IP
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0070	Anti-Mouse CD8a (Ly 2.2) In Vivo Antibody - Low Endotoxin	Low
IVMB0071	Anti-Mouse CD8a (Ly 2.2) In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	Depletion, FA, FC, ICC, IF Staining, IHC FFPE, IP
Recommended Usage:	FC The suggested concentration for this 2.43 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 10⁶ in a volume of 100 µl. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:	Mouse

Host Species:	Rat
Specificity:	Clone 2.43 recognizes an epitope on mouse CD8a.
Antigen Distribution:	CD8a is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic-cells.
Immunogen:	Mouse CTL clone L3
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD8 is made up of disulfide-linked α and ? chains that form the α(CD8a)/?(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.

Protein:	CD8a
Function:	Depleting activity when used in vivo.
Ligand/Receptor:	MHC class I molecule

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