Anti-Mouse H-2Db (Clone B22/249) In Vivo Antibody

Product Name:	Anti-Mouse H-2Db (Clone B22/249) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0257
Size:	1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:	B22/249
Product Type:	Hybridoma Monoclonal Antibody
Synonyms:	H-2D; H2-D; H2-D1
Isotype:	Mouse IgG2a k
Reactivity:	Mouse
Applications:	Cytotoxicity Assay, In vitro depletion, IP
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:	≤ 1.0 EU/mg as determined by the LAL method
Purity:	≥95% by SDS Page ⋅ ≥95% monomer by analytical SEC
Product Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Applications:	Cytotoxicity Assay, In vitro depletion, IP
Reactivity:	Mouse

Host Species:	Mouse
Specificity:	B22.249.R1 activity is directed against mouse H-2Db.
Antigen Distribution:	H-2Db is expressed on the cell surface of lymphocytes.
Immunogen:	Mouse H-2Db
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	≤ 1.0 EU/mg as determined by the LAL method
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:	≥95% by SDS Page ⋅ ≥95% monomer by analytical SEC
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response1. H-2Db is a class I MHC cell surface protein. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins, and then display those peptides on the cell surface. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. For example, H-2Db plays a role in human papillomavirus infection2. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell1.

B22-249.R1 was generated by immunizing mice with spleen cells from allogeneic mice using the combination BSLB/k anti-C57BL/63. Spleen cells of the recipient mouse were hybridized with the P3-NS 1-Ag4 myeloma cell line and screened using cytotoxic and hemagglutination assays. B22-249.R1 is also known as H-2.m2 in the literature4.

Ligand/Receptor:	CD3/TCR, CD8
Research Area:	Immunology
State of Matter:	Liquid
Shipping:	2-8°C
NCBI Gene Bank ID:	14964