Description
Anti-Mouse H-2Kd (MHC Class I) In Vivo Antibody - Low Endotoxin
Introducing the Anti-Mouse H-2Kd MHC Class I In Vivo Antibody - Low Endotoxin from Assay Genie, a highly specific monoclonal antibody tailored for in vivo applications.
This antibody targets the H-2Kd molecule, an important component in the major histocompatibility complex (MHC) class I that is essential for presenting endogenous antigens to CD8+ T-cells, thereby playing a pivotal role in immune surveillance and response. Featuring a rat IgG2a kappa isotype, this antibody ensures high purity and low endotoxin levels (<1.0 EU/mg), making it ideal for applications such as ELISA, flow cytometry, immunohistochemistry, and other relevant assays. Available in various sizes, it is formulated in phosphate-buffered saline to ensure optimal stability and efficacy. Enhance your immunological research with this reliable and versatile antibody. H-2Kd is a glycoprotein expressed on the surface of nucleated cells in mice, contributing to the presentation of antigenic peptides to cytotoxic T lymphocytes (CTLs). This interaction is crucial for the immune system to recognize and eliminate cells expressing foreign antigens, such as those infected by pathogens or transformed into cancer cells.
| Product Name: | Anti-Mouse H-2Kd (MHC Class I) In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0178 |
| Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
| Clone: | A4C8.1-Do9 |
| Protein: | MHC Class I |
| Product Type: | Monoclonal Antibody |
| Synonyms: | Class I major histocompatibility antigen H-2Kd |
| Isotype: | Mouse IgG1 κ |
| Reactivity: | Mouse |
| Applications: | FC, WB |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Applications: | FC, WB |
| Recommended Usage: | FC The suggested concentration for clone A4C8.1-Do9 antibody for staining cells in flow cytometry is ≤ .25 µg per 106 in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. |
| Reactivity: | Mouse |
| Host Species: | Mouse |
| Specificity: | Clone A4C8.1-Do9 recognizes an epitope on mouse MHC class I H-2K haplotype d. |
| Antigen Distribution: | H-2Kd is ubiquitously expressed on nucleated cells from mice of the H-2Kd haplotype, including BALB/c. |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
H-2Kd antibody, clone A4C8.1-Do9, recognizes the major histocompatibility complex (MHC) class I H-2K haplotype d (H-2Kd). MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2Kd plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.
| Datasheet: | View |
| Protein: | MHC Class I |
| Research Area: | Immunology, Innate Immunity |
| Mouse IgG1 Isotype Control [HKSP84] | |
|---|---|
| Clone | HKSP84 |
| Isotype | Mouse IgG1 |
| Endotoxin Level | Low Endotoxin |
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