Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody

Product Name:	Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0010
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	Y-3
Protein:	MHC Class I
Product Type:	Monoclonal Antibody
Synonyms:	H-2K, H-2 class I histocompatibility antigen, K-B alpha chain, H-2K(B), H-2K(K), H-2K(Q), H-2K(R), H-2K(S), Beta-2-microglobulin
Isotype:	Mouse IgG2b κ
Reactivity:	Mouse
Immunogen:	Con A stimulated spleen cells from BALB.B mice
Applications:	FA, ICC, IP, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0009	Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Low Endotoxin	Low
IVMB0010	Anti-Mouse H-2Kb (MHC Class I) [Y-3] In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	FA, ICC, IP, WB
Reactivity:	Mouse

Host Species:	Mouse
Specificity:	Clone Y-3 recognizes an epitope on mouse MHC class I H-2K haplotypes b, k, q, r, s, but not d.
Antigen Distribution:	H-2K is ubiquitously expressed on nucleated cells.
Immunogen:	Con A stimulated spleen cells from BALB.B mice
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	<0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

H-2K antibody, clone Y-3, recognizes the major histocompatibility complex (MHC) class I H-2K haplotypes b, k, q, r, s, but not d. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2K plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.

Technical Datasheet:	View
Protein:	MHC Class I
Ligand/Receptor:	CD3/TCR, CD4
Research Area:	Immunology, Innate Immunity

Mouse IgG2b Isotype Control
Clone	MPC-11
Isotype	Mouse IgG2b
Endotoxin Level	Ultra Low Endotoxin

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