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Anti-MYH6 Antibody (CAB12964)

SKU:
CAB12964
Product Type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 3 Anti-MYH6 Antibody CAB12964
  • Cell Biology Antibodies 3 Anti-MYH6 Antibody CAB12964
€139 - €419
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Description

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Antibody Name:Anti-MYH6 Antibody
Antibody SKU:CAB12964
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MYH6 (NP_002462.2).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MYH6 (NP_002462.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTDA QMAD FGAA AQYL RKSE KERL EAQT RPFD IRTE CFVP DDKE EFVK AKIL SREG GKVI AETE NGKT VTVK EDQV LQQN PPKF DKIE DMAM LTFL HEPA VLFN LKER YAAW MIYT YSGL FCVT VNPY KWLP VYNA EVVA AYRG KKRS EAPP HIFS ISDN AYQY MLTD RENQ SILI TGES GAGK TVNT KRVI QYFA SIAA IGDR GKKD NANA NKGT LEDQ IIQA NPAL EAFG NAKT VRND NSSR FGKF IR
Gene ID:4624
Uniprot:P13533
Cellular Location:Cytoplasm, myofibril
Calculated MW:223kDa
Observed MW:250kDa
Synonyms:MYH6, ASD3, CMD1EE, CMH14, MYHC, MYHCA, SSS3, alpha-MHC, myosin-6
Background:Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3.
UniProt Protein Function:MYH6: Muscle contraction. Defects in MYH6 are the cause of atrial septal defect type 3 (ASD3). ASD3 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Defects in MYH6 are the cause of familial hypertrophic cardiomyopathy type 14 (CMH14). It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations,and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in MYH6 are the cause of cardiomyopathy dilated type 1EE (CMD1EE). It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in MYH6 are the cause of susceptibility to sick sinus syndrome type 3 (SSS3). The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors. MYH6 variations are associated with susceptibility to sick sinus syndrome (PubMed:21378987). The lifetime risk of being diagnosed with sick sinus syndrome is higher for carriers of variant p.Arg721Trp than for non-carriers (PubMed:21378987).
UniProt Protein Details:

Protein type:Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytosol; muscle myosin complex; myofibril; myosin complex; sarcomere

Molecular Function:actin-dependent ATPase activity; ATPase activity; microfilament motor activity; myosin phosphatase activity; protein kinase binding

Biological Process: adult heart development; ATP metabolic process; atrial cardiac muscle morphogenesis; cardiac muscle contraction; cardiac muscle fiber development; in utero embryonic development; muscle contraction; muscle filament sliding; myofibril assembly; regulation of ATPase activity; regulation of blood pressure; regulation of heart contraction; regulation of heart rate; regulation of the force of heart contraction; sarcomere organization; striated muscle contraction; ventricular cardiac muscle morphogenesis; visceral muscle development

Disease: Atrial Septal Defect 3; Cardiomyopathy, Dilated, 1ee; Cardiomyopathy, Familial Hypertrophic, 1; Cardiomyopathy, Familial Hypertrophic, 14; Sick Sinus Syndrome 3, Susceptibility To

NCBI Summary:Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located ~4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. [provided by RefSeq, Mar 2010]
UniProt Code:P13533
NCBI GenInfo Identifier:317373582
NCBI Gene ID:4624
NCBI Accession:P13533.5
UniProt Secondary Accession:P13533,Q13943, Q14906, Q14907, A2RTX1, D9YZU2,
UniProt Related Accession:P13533
Molecular Weight:223,735 Da
NCBI Full Name:Myosin-6
NCBI Synonym Full Names:myosin heavy chain 6
NCBI Official Symbol:MYH6  
NCBI Official Synonym Symbols:ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC  
NCBI Protein Information:myosin-6
UniProt Protein Name:Myosin-6
UniProt Synonym Protein Names:Myosin heavy chain 6; Myosin heavy chain, cardiac muscle alpha isoform; MyHC-alpha
Protein Family:Myosin
UniProt Gene Name:MYH6  
UniProt Entry Name:MYH6_HUMAN
Anti-MYH6 Antibody (CAB12964)Western blot analysis of extracts of Mouse skeletal muscle, using MYH6 Rabbit pAb (CAB12964) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-MYH6 Antibody (CAB12964)Immunofluorescence analysis of rat heart using MYH6 antibody (CAB12964) at dilution of 1:100. Blue: DAPI for nuclear staining.

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