Anti-MYH7 Antibody (CAB7564)

Antibody Name:	Anti-MYH7 Antibody
Antibody SKU:	CAB7564
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1766-1935 of human MYH7 (NP_000248.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse heart, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1766-1935 of human MYH7 (NP_000248.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AEEL KKEQ DTSA HLER MKKN MEQT IKDL QHRL DEAE QIAL KGGK KQLQ KLEA RVRE LENE LEAE QKRN AESV KGMR KSER RIKE LTYQ TEED RKNL LRLQ DLVD KLQL KVKA YKRQ AEEA EEQA NTNL SKFR KVQH ELDE AEER ADIA ESQV NKLR AKSR DIGT KGLN EE
Gene ID:	4625
Uniprot:	P12883
Cellular Location:	Cytoplasm, myofibril
Calculated MW:	223kDa
Observed MW:	220KDa

Synonyms:

MYH7, CMD1S, CMH1, MPD1, MYHCB, SPMD, SPMM, myosin-7, MYH7

Background:

Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy.

UniProt Protein Function:	MYH7: Muscle contraction. Defects in MYH7 are the cause of familial hypertrophic cardiomyopathy type 1 (CMH1). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in MYH7 are the cause of myopathy myosin storage (MYOMS). In this disorder, muscle biopsy shows type 1 fiber predominance and increased interstitial fat and connective tissue. Inclusion bodies consisting of the beta cardiac myosin heavy chain are present in the majority of type 1 fibers, but not in type 2 fibers. Defects in MYH7 are the cause of scapuloperoneal myopathy MYH7-related (SPMM); also known as scapuloperoneal syndrome myopathic type. SPMM is a progressive muscular atrophia beginning in the lower legs and affecting the shoulder region earlier and more severely than distal arm. Defects in MYH7 are a cause of cardiomyopathy dilated type 1S (CMD1S). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in MYH7 are the cause of myopathy distal type 1 (MPD1). MPD1 is a muscular disorder characterized by early-onset selective weakness of the great toe and ankle dorsiflexors, followed by weakness of the finger extensors. Mild proximal weakness occasionally develops years later after the onset of the disease.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 14q12 Cellular Component: nucleoplasm; sarcomere; focal adhesion; cytoplasm; stress fiber; muscle myosin complex; myosin complex; Z disc Molecular Function:microfilament motor activity; calmodulin binding; protein binding; ATPase activity; actin-dependent ATPase activity; actin binding; ATP binding Biological Process: adult heart development; striated muscle contraction; muscle contraction; regulation of heart rate; metabolic process; ventricular cardiac muscle morphogenesis; regulation of the force of heart contraction; muscle filament sliding Disease: Myopathy, Distal, 1; Scapuloperoneal Myopathy, Myh7-related; Myopathy, Myosin Storage; Cardiomyopathy, Dilated, 1s; Cardiomyopathy, Familial Hypertrophic, 1; Myopathy, Myosin Storage, Autosomal Recessive; Myopathy, Congenital, With Fiber-type Disproportion
NCBI Summary:	Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008]
UniProt Code:	P12883
NCBI GenInfo Identifier:	83304912
NCBI Gene ID:	4625
NCBI Accession:	P12883.5
UniProt Secondary Accession:	P12883,Q14836, Q14837, Q14904, Q16579, Q2M1Y6, Q92679 Q9H1D5, Q9UDA2, Q9UMM8, A2TDB6, B6D424,
UniProt Related Accession:	P12883
Molecular Weight:	223,097 Da
NCBI Full Name:	Myosin-7
NCBI Synonym Full Names:	myosin, heavy chain 7, cardiac muscle, beta
NCBI Official Symbol:	MYH7
NCBI Official Synonym Symbols:	CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
NCBI Protein Information:	myosin-7
UniProt Protein Name:	Myosin-7
UniProt Synonym Protein Names:	Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; Myosin heavy chain, cardiac muscle beta isoform; MyHC-beta
Protein Family:	Myosin
UniProt Gene Name:	MYH7
UniProt Entry Name:	MYH7_HUMAN

	Western blot analysis of extracts of Rat heart, using MYH7 Rabbit pAb (CAB7564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of Mouse heart, using MYH7 Rabbit pAb (CAB7564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded mouse skeletal muscle using MYH7 antibody (CAB7564) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using MYH7 antibody (CAB7564) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of rat skeletal muscle using MYH7 antibody (CAB7564) at dilution of 1:100. Blue: DAPI for nuclear staining.

ELISA Kits

Human Myosin-7 (MYH7) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)