The PAX5 Monoclonal Antibody (CAB9607) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this polyclonal antibody exhibits excellent reactivity with human samples and is validated for use in various applications including Western blot and immunohistochemistry.PAX5, also known as B-cell specific activator protein (BSAP), plays a crucial role in B-cell differentiation and maturation, as well as in the regulation of other genes involved in B-cell development.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PAX5 Monoclonal Antibody
SKU:
CAB9607
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1654
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ALL3, BSAP, PAX-5, PAX5
Positive Sample:
Raji
Cellular Localization:
Nuclear.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
5079
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Raji cells, using PAX5 Rabbit mAb (CAB9607) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human spleen using PAX5 Rabbit mAb (CAB9607) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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