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Anti-Timm22 Antibody (CAB20813)

SKU:
CAB20813
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Reactivity:
Mouse
Rat
Host Species:
Goat
Isotype:
IgG
  • Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug Timm22 antibody . Western blot was performed from the immunoprecipitate using Timm22 antibody at a dilition of 1:500.
  • Western blot analysis of extracts of various cell lines, using Timm22 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
€139 - €419
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Description

Antibody Name:Anti-Timm22 Antibody (CAB20813)
Antibody SKU:CAB20813
Antibody Size:50µL, 100µL
Application:Western blotting, Immunoprecipitation
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-121 of mouse Timm22 (NP_062792.2).
Application:Western blotting, Immunoprecipitation
Recommended Dilution:WB 1:500 - 1:2000
IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:T47D, Mouse brain, Rat testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-121 of mouse Timm22 (NP_062792.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AEAP LQYS LLLQ YLVG DKRQ PRLL EPGS LGGI PSPA KSEE QKMI ERAM ESCA FKAV LACV GGFV LGGA FGIF TAGI DTNV GFDP KDPY RTPT AKEV LKDM GQR
Cellular Location:
Calculated MW:20kDa
Observed MW:20KDa
Synonyms:Tim22
Background:Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps (By similarity.
Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug Timm22 antibody . Western blot was performed from the immunoprecipitate using Timm22 antibody at a dilition of 1:500.Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug Timm22 antibody . Western blot was performed from the immunoprecipitate using Timm22 antibody at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using Timm22 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using Timm22 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

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