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Timm22 Polyclonal Antibody (CAB20813)

SKU:
CAB20813
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Reactivity:
Mouse
Rat
Host Species:
Goat
Isotype:
IgG
  • Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug Timm22 antibody . Western blot was performed from the immunoprecipitate using Timm22 antibody at a dilition of 1:500.
  • Western blot analysis of extracts of various cell lines, using Timm22 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
€139 - €419
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Description

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Product Name:Timm22 Polyclonal Antibody
SKU:CAB20813
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 19-121 of mouse Timm22 (NP_062792.2).
Sequence:AEAP LQYS LLLQ YLVG DKRQ PRLL EPGS LGGI PSPA KSEE QKMI ERAM ESCA FKAV LACV GGFV LGGA FGIF TAGI DTNV GFDP KDPY RTPT AKEV LKDM GQR
Tested Applications:WB IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:Tim22; Timm22
Positive Sample:T47D,Mouse brain,Rat testis,Rat brain
Conjugate:Unconjugated
Cellular Localization:mitochondrial inner membrane, mitochondrion
Calculated MW:20kDa
Observed MW:20kDa

Predicted to enable mitochondrion targeting sequence binding activity and protein transmembrane transporter activity. Predicted to be involved in protein insertion into mitochondrial inner membrane. Predicted to act upstream of or within protein transport. Located in mitochondrion. Is expressed in several structures, including alimentary system; brain; cardiovascular system; egg cylinder; and genitourinary system. Human ortholog(s) of this gene implicated in combined oxidative phosphorylation deficiency 43. Orthologous to human TIMM22 (translocase of inner mitochondrial membrane 22).

Purification Method:Affinity purification
Gene ID:56322
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Timm22 Rabbit pAb (CAB20813) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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