The TIRAP Monoclonal Antibody (CAB9663) is a high-quality antibody developed for reliable detection and analysis of target proteins. TIRAP is a key adaptor protein involved in Toll-like receptor (TLR) signaling pathways, which play a crucial role in the innate immune response.This polyclonal antibody, generated in rabbits, is designed for use in various research applications, including Western blotting, immunofluorescence, and immunohistochemistry. It specifically targets TIRAP, allowing for the detection and analysis of TIRAP protein levels and localization in different cell types and tissues.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TIRAP Monoclonal Antibody
SKU:
CAB9663
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1686
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Mal, wyatt, BACTS1, MyD88-2, TIRAP
Positive Sample:
293T, Hep G2, THP-1, Mouse brain, Rat spleen, Rat thymus
Cellular Localization:
Cell Membrane, Cytoplasm, Membrane.
Calculated MW:
24kDa
Observed MW:
32kDa
The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.
Purification Method
Affinity purification
Gene ID
114609
RRID
AB_2863753
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TIRAP Rabbit mAb (CAB9663) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat lung using TIRAP Rabbit mAb (CAB9663) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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