Anti-AP2A1 Antibody (CAB6863)

Antibody Name:	Anti-AP2A1 Antibody
Antibody SKU:	CAB6863
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human AP2A1 (NP_055018.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, HeLa, Rat liver, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human AP2A1 (NP_055018.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPAV SKGD GMRG LAVF ISDI RNCK SKEA EIKR INKE LANI RSKF KGDK ALDG YSKK KYVC KLLF IFLL GHDI DFGH MEAV NLLS SNKY TEKQ IGYL FISV LVNS NSEL IRLI NNAI KNDL ASRN PTFM CLAL HCIA NVGS REMG EAFA ADIP RILV AGDS MDSV KQSA ALCL LRLY KASP DLVP MGEW TARV VHLL NDQH MGVV TAAV SLIT CLCK KNPD DFKT CVSL AVSR LSRI VSSA STDL QDYT YYFV PAPW LSVK
Gene ID:	160
Uniprot:	O95782
Cellular Location:	Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit
Calculated MW:	105kDa/107kDa
Observed MW:	108kDa

Synonyms:

AP2A1, ADTAA, AP2-ALPHA, CLAPA1

Background:

This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.

UniProt Protein Function:	Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
NCBI Summary:	This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	O95782
NCBI GenInfo Identifier:	262527580
NCBI Gene ID:	160
NCBI Accession:	O95782.3
UniProt Secondary Accession:	O95782,Q96CI7, Q96PP6, Q96PP7, Q9H070,
UniProt Related Accession:	O95782
Molecular Weight:	105,361 Da
NCBI Full Name:	AP-2 complex subunit alpha-1
NCBI Synonym Full Names:	adaptor related protein complex 2 subunit alpha 1
NCBI Official Symbol:	AP2A1
NCBI Official Synonym Symbols:	ADTAA; CLAPA1; AP2-ALPHA
NCBI Protein Information:	AP-2 complex subunit alpha-1
UniProt Protein Name:	AP-2 complex subunit alpha-1
UniProt Synonym Protein Names:	100 kDa coated vesicle protein A; Adaptor protein complex AP-2 subunit alpha-1; Adaptor-related protein complex 2 subunit alpha-1; Alpha-adaptin A; Alpha1-adaptin; Clathrin assembly protein complex 2 alpha-A large chain; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
Protein Family:	AP-2 complex
UniProt Gene Name:	AP2A1

	Western blot analysis of extracts of various cell lines, using AP2A1 antibody (CAB6863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat kidney using AP2A1 Antibody (CAB6863) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using AP2A1 Antibody (CAB6863) at dilution of 1:100 (40x lens).

Antibodies

Anti-Alpha1 adaptin Antibody (CAB4403)

AP2A1 Antibody (PACO07766)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)