The APCDD1 Polyclonal Antibody (PAC063443) is a valuable tool for researchers studying APCDD1, a protein involved in the Wnt signaling pathway, which plays a critical role in various cellular processes such as cell proliferation, differentiation, and migration. This polyclonal antibody, generated in rabbits, is highly specific and reactive with human samples, making it suitable for Western blot applications.APCDD1 is known for its function as a negative regulator of the Wnt signaling pathway, acting as a tumor suppressor in certain cancers. Its involvement in cell growth and development makes it a target of interest in cancer research and developmental biology.
By targeting APCDD1 with this antibody, researchers can detect and analyze its expression levels in different cell types, shedding light on its role in disease progression and potential therapeutic interventions.Furthermore, understanding the function of APCDD1 can provide insights into its contribution to various diseases, such as colorectal cancer and Alzheimer's disease. By studying APCDD1 with the PAC063443 antibody, researchers can further explore its mechanisms of action and potentially uncover new targets for drug development and treatment strategies.
Western Blot. Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate. All lanes: APCDD1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59 kDa. Observed band size: 59 kDa.
IHC image of PACO63443 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO63443 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Negative regulator of the Wnt signaling pathway. Inhibits Wnt signaling in a cell-autonomous manner and functions upstream of beta-catenin. May act via its interaction with Wnt and LRP proteins. May play a role in colorectal tumorigenesis.
Synonyms:
Protein APCDD1 (Adenomatosis polyposis coli down-regulated 1 protein), APCDD1, DRAPC1
UniProt Protein Function:
APCDD1: a single-pass type I membrane protein. Probably plays a role in colorectal tumorigenesis. Directly activated downstream targets of the WNT/beta-catenin pathway. Transcriptional target of beta-catenin/T-cell (Tcf) factor and may enhance cellular proliferation of tumor cells. Down-regulated by wild-type the adenomatosis polyposis coli (APC) protein. Elevated in many primary colon cancer tissues.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 18p11.22Cellular Component: integral to plasma membraneMolecular Function: Wnt-protein bindingBiological Process: hair follicle development; negative regulation of Wnt receptor signaling pathwayDisease: Hypotrichosis 1
UniProt Protein Details:
NCBI Summary:
This locus encodes an inhibitor of the Wnt signaling pathway. Mutations at this locus have been associated with hereditary hypotrichosis simplex. Increased expression of this gene may also be associated with colorectal carcinogenesis.[provided by RefSeq, Sep 2010]
