Anti-APOA1 Antibody (CAB14211)

Antibody Name:	Anti-APOA1 Antibody
Antibody SKU:	CAB14211
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human APOA1 (NP_000030.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse lung, mouse liver, mouse small intestine, rat lung, rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-267 of human APOA1 (NP_000030.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DEPP QSPW DRVK DLAT VYVD VLKD SGRD YVSQ FEGS ALGK QLNL KLLD NWDS VTST FSKL REQL GPVT QEFW DNLE KETE GLRQ EMSK DLEE VKAK VQPY LDDF QKKW QEEM ELYR QKVE PLRA ELQE GARQ KLHE LQEK LSPL GEEM RDRA RAHV DALR THLA PYSD ELRQ RLAA RLEA LKEN GGAR LAEY HAKA TEHL STLS EKAK PALE DLRQ GLLP VLES FKVS FLSA LEEY TKKL NTQ
Gene ID:	335
Uniprot:	P02647
Cellular Location:	Secreted
Calculated MW:	30kDa
Observed MW:	31kDa

Synonyms:

APOA1, apo(a)

Background:

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.

UniProt Protein Function:	APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.
UniProt Protein Details:	Protein type:Lipid-binding; Secreted, signal peptide; Secreted; Vesicle; Motility/polarity/chemotaxis; Cell development/differentiation; Endoplasmic reticulum Chromosomal Location of Human Ortholog: 11q23-q24 Cellular Component: extracellular space; chylomicron; cell surface; endocytic vesicle; endoplasmic reticulum lumen; early endosome; extracellular region; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; vesicle Molecular Function:identical protein binding; protein binding; enzyme binding; phospholipid transporter activity; lipase inhibitor activity; beta-amyloid binding; chemorepellent activity; cholesterol transporter activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; apolipoprotein A-I receptor binding; high-density lipoprotein binding; apolipoprotein receptor binding Biological Process: phototransduction, visible light; negative chemotaxis; negative regulation of lipase activity; axon regeneration in the peripheral nervous system; sequestering of lipid; negative regulation of interleukin-1 beta secretion; regulation of cholesterol absorption; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; response to drug; platelet activation; cholesterol metabolic process; organ regeneration; regulation of Cdc42 protein signal transduction; adrenal gland development; positive regulation of hydrolase activity; positive regulation of Rho protein signal transduction; lipoprotein metabolic process; positive regulation of transferase activity; vitamin transport; cholesterol biosynthetic process; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; lipoprotein biosynthetic process; response to estrogen stimulus; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; positive regulation of lipoprotein lipase activity; blood vessel endothelial cell migration; cellular lipid metabolic process; platelet degranulation; phospholipid efflux; retinoid metabolic process; transmembrane transport; response to nutrient; phospholipid homeostasis; integrin-mediated signaling pathway; receptor-mediated endocytosis; positive regulation of fatty acid biosynthetic process; regulation of protein amino acid phosphorylation; cholesterol transport; protein stabilization; protein amino acid oxidation; negative regulation of heterotypic cell-cell adhesion; neurite regeneration; cholesterol efflux; G-protein coupled receptor protein signaling pathway; glucocorticoid metabolic process; reverse cholesterol transport; endothelial cell proliferation; negative regulation of inflammatory response; blood coagulation Disease: Hypoalphalipoproteinemia, Primary; Amyloidosis, Familial Visceral
NCBI Summary:	This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]
UniProt Code:	P02647
NCBI GenInfo Identifier:	113992
NCBI Gene ID:	335
NCBI Accession:	P02647.1
UniProt Secondary Accession:	P02647,Q6LDN9, Q6Q785, Q9UCS8, Q9UCT8, A8K866,
UniProt Related Accession:	P02647
Molecular Weight:	30,778 Da
NCBI Full Name:	Apolipoprotein A-I
NCBI Synonym Full Names:	apolipoprotein A-I
NCBI Official Symbol:	APOA1
NCBI Protein Information:	apolipoprotein A-I; apo-AI
UniProt Protein Name:	Apolipoprotein A-I
UniProt Synonym Protein Names:	Apolipoprotein A1Cleaved into the following 2 chains:Proapolipoprotein A-I; ProapoA-I; Truncated apolipoprotein A-IAlternative name(s):Apolipoprotein A-I(1-242)
Protein Family:	Apolipoprotein
UniProt Gene Name:	APOA1
UniProt Entry Name:	APOA1_HUMAN

	Western blot analysis of extracts of various cell lines, using APOA1 antibody (CAB14211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded mouse liver using APOA1 antibody (CAB14211) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using APOA1 antibody (CAB14211) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using APOA1 antibody (CAB14211) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-APOA1 Antibody (CAB1129)	Human Apolipoprotein A-I / APOA1 ELISA Kit
APOA1 Antibody (PACO02115)	Human ApoA1 (Apolipoprotein A1) CLIA Kit (HUES00116)
APOA1 Antibody (PACO07786)	Human ApoA1 (Apolipoprotein A1) ELISA Kit (HUES01407)
	Human Apolipoprotein A-I (APOA1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)