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Anti-APOB Antibody (CAB1330)

SKU:
CAB1330
Product Type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 1 Anti-APOB Antibody CAB1330
  • Metabolism Antibodies 1 Anti-APOB Antibody CAB1330
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-APOB Antibody
Antibody SKU:CAB1330
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-330 of human APOB (NP_000375.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:LO2, HepG2, HL-60, Mouse liver, Mouse kidney, Rat liver, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-330 of human APOB (NP_000375.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EEEM LENV SLVC PKDA TRFK HLRK YTYN YEAE SSSG VPGT ADSR SATR INCK VELE VPQL CSFI LKTS QCTL KEVY GFNP EGKA LLKK TKNS EEFA AAMS RYEL KLAI PEGK QVFL YPEK DEPT YILN IKRG IISA LLVP PETE EAKQ VLFL DTVY GNCS THFT VKTR KGNV ATEI STER DLGQ CDRF KPIR TGIS PLAL IKGM TRPL STLI SSSQ SCQY TLDA KRKH VAEA ICKE QHLF LPFS YKNK YGMV AQVT QTLK LEDT PKIN SRFF GEGT KKMG LAFE STKS TSPP KQAE AVLK TLQ
Gene ID:338
Uniprot:P04114
Cellular Location:Cytoplasm, Secreted
Calculated MW:515kDa
Observed MW:Refer to figures
Synonyms:APOB, FLDB, LDLCQ4, apoB-100, apoB-48
Background:This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
UniProt Protein Function:Function: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
UniProt Protein Details:

Subcellular location: Secreted.

Induction: Up-regulated in response to enterovirus 71 (EV71) infection (at protein level). Ref.34

Post-translational modification: Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. Ref.31

Involvement in Disease: Defects in APOB are a cause of hypobetalipoproteinemia familial type 1 (FHBL1) [

MIM:107730]. A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Ref.46Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB) [

MIM:144010]. FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Ref.40 Ref.42 Ref.44Note=Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Sequence similarities: Contains 1 vitellogenin domain.

RNA editing: Edited at position 2180.The stop codon (UAA) at position 2180 is created by RNA editing. Apo B-48, derived from the fully edited RNA, is produced only in the intestine and is found in chylomicrons. Apo B-48 is a shortened form of apo B-100 which lacks the LDL-receptor region. The unedited version (apo B-100) is produced by the liver and is found in the VLDL and LDL.

Sequence caution: The sequence AAA51752.1 differs from that shown. Reason: Frameshift at positions 942, 951, 1139, 1165, 1164, 1371 and 1385.

NCBI Summary:This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq]
UniProt Code:P04114
NCBI GenInfo Identifier:114014
NCBI Gene ID:338
NCBI Accession:P04114.1
UniProt Secondary Accession:P04114,O00502, P78479, P78480, P78481, Q13779, Q13785 Q13786, Q13787, Q13788, Q4ZG63, Q53QC8,
UniProt Related Accession:P04114,P78482,Q13789,Q13828,Q59HB3,Q7Z7Q0,Q9UE51,Q9UE52,Q9UE53
Molecular Weight:
NCBI Full Name:Apolipoprotein B-100
NCBI Synonym Full Names:apolipoprotein B (including Ag(x) antigen)
NCBI Official Symbol:APOB  
NCBI Official Synonym Symbols:FLDB; LDLCQ4  
NCBI Protein Information:apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; OTTHUMP00000115994; apolipoprotein B48
UniProt Protein Name:Apolipoprotein B-100
Protein Family:Apolipoprotein
UniProt Gene Name:APOB  
UniProt Entry Name:APOB_HUMAN
Anti-APOB Antibody (CAB1330)Immunofluorescence analysis of C6 cells using APOB antibody (CAB1330) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-APOB Antibody (CAB1330)Immunofluorescence analysis of U-2 OS cells using APOB antibody (CAB1330) at dilution of 1:100. Blue: DAPI for nuclear staining.

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