Anti-APOBEC3A Antibody (CAB12399)

Antibody Name:	Anti-APOBEC3A Antibody
Antibody SKU:	CAB12399
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human APOBEC3A (NP_663745.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	HT-29

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human APOBEC3A (NP_663745.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEAS PASG PRHL MDPH IFTS NFNN GIGR HKTY LCYE VERL DNGT SVKM DQHR GFLH NQAK NLLC GFYG RHAE LRFL DLVP SLQL DPAQ IYRV TWFI SWSP CFSW GCAG EVRA FLQE NTHV RLRI FAAR IYDY DPLY KEAL QMLR DAGA QVSI MTYD EFKH CWDT FVDH QGCP FQPW DGLD EHSQ ALSG RLRA ILQN QGN
Gene ID:	200315
Uniprot:	P31941
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	21kDa/23kDa
Observed MW:	26kDa

Synonyms:

APOBEC3A, A3A, ARP3, PHRBN, bK150C2.1

Background:

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	APOBEC3A: a single-stranded DNA cytidine deaminase involved in foreign DNA clearance with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic C-to-U hypermutation in nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Interacts with AGO2. Interacts with TRIB3. Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia. Up-regulated by interferon and CpG single-stranded DNA (at protein level). Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:	Protein type:EC 3.5.4.-; Hydrolase Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: cytoplasm; nucleus Molecular Function:cytidine deaminase activity; deoxycytidine deaminase activity; protein binding Biological Process: defense response to virus; innate immune response; negative regulation of viral genome replication
NCBI Summary:	This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:	P31941
NCBI GenInfo Identifier:	12644206
NCBI Gene ID:	200315
NCBI Accession:	P31941.3
UniProt Secondary Accession:	P31941,Q12807, Q5JZ93, Q9UH18, A0AVM1,
UniProt Related Accession:	P31941
Molecular Weight:	23kDa
NCBI Full Name:	DNA dC->
NCBI Synonym Full Names:	apolipoprotein B mRNA editing enzyme catalytic subunit 3A
NCBI Official Symbol:	APOBEC3A
NCBI Official Synonym Symbols:	A3A; ARP3; PHRBN; bK150C2.1
NCBI Protein Information:	DNA dC->dU-editing enzyme APOBEC-3A
UniProt Protein Name:	DNA dC->dU-editing enzyme APOBEC-3A
UniProt Synonym Protein Names:	Phorbolin-1
Protein Family:	DNA dC->dU-editing enzyme
UniProt Gene Name:	APOBEC3A

Western blot analysis of extracts of HT-29 cells, using APOBEC3A antibody (CAB12399) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

APOBEC3A Antibody (PACO02638)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)