Anti-APOBEC3B Antibody (CAB9010)

Antibody Name:	Anti-APOBEC3B Antibody
Antibody SKU:	CAB9010
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APOBEC3B (NP_004891.4).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:1000
Reactivity:	Human
Positive Samples:	HeLa, U-251MG, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APOBEC3B (NP_004891.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNPQ IRNP MERM YRDT FYDN FENE PILY GRSY TWLC YEVK IKRG RSNL LWDT GVFR GQVY FKPQ YHAE MCFL SWFC GNQL PAYK CFQI TWFV SWTP CPDC VAKL AEFL SEHP NVTL TISA ARLY YYWE RDYR RALC RLSQ AGAR VKIM DYEE FAYC WENF VYNE GQQF MPWY KFDE NYAF
Gene ID:	9582
Uniprot:	Q9UH17
Cellular Location:	Nucleus
Calculated MW:	29kDa/43kDa/45kDa
Observed MW:	46kDa

Synonyms:

APOBEC3B, A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2

Background:

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A hybrid gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3B, and the adjacent gene APOBEC3A. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	APOBEC3B: a DNA cytidine deaminase involved in foreign DNA clearance. Acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Homodimer. Interacts with APOBEC3G. Does not interact with APOBEC1. Expressed at high and moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate and ovary. Also expressed at low levels in several other tissues. Phorbol 12-myristate 13-acetate (PMA) induces overexpression in keratinocytes. Up-regulated by IFN-alpha. Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.5.4.-; RNA-binding; Hydrolase Chromosomal Location of Human Ortholog: 22q13.1-q13.2 Cellular Component: nucleus Molecular Function:deoxycytidine deaminase activity Biological Process: defense response to virus
NCBI Summary:	This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A hybrid gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3B, and the adjacent gene APOBEC3A. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:	Q9UH17
NCBI GenInfo Identifier:	12643884
NCBI Gene ID:	9582
NCBI Accession:	Q9UH17.1
UniProt Secondary Accession:	Q9UH17,O95618, Q20WL1, Q5IFJ4, Q7Z2N3, Q7Z6D6, Q9UE74 B0QYD2,
UniProt Related Accession:	Q9UH17
Molecular Weight:	43,081 Da
NCBI Full Name:	DNA dC->
NCBI Synonym Full Names:	apolipoprotein B mRNA editing enzyme catalytic subunit 3B
NCBI Official Symbol:	APOBEC3B
NCBI Official Synonym Symbols:	A3B; ARP4; ARCD3; PHRBNL; APOBEC1L; bK150C2.2; DJ742C19.2
NCBI Protein Information:	DNA dC->dU-editing enzyme APOBEC-3B
UniProt Protein Name:	DNA dC->dU-editing enzyme APOBEC-3B
UniProt Synonym Protein Names:	Phorbolin-1-related protein; Phorbolin-2/3
Protein Family:	DNA dC->dU-editing enzyme
UniProt Gene Name:	APOBEC3B
UniProt Entry Name:	ABC3B_HUMAN

Anti-APOBEC3B Polyclonal Antibody (CAB9010)

Western blot analysis of extracts of various cell lines, using APOBEC3B antibody (CAB9010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)