Anti-APOBEC3G Antibody (CAB13598)

Antibody Name:	Anti-APOBEC3G Antibody
Antibody SKU:	CAB13598
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-330 of human APOBEC3G (NP_068594.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	PC-3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-330 of human APOBEC3G (NP_068594.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SELK YHPE MRFF HWFS KWRK LHRD QEYE VTWY ISWS PCTK CTRD MATF LAED PKVT LTIF VARL YYFW DPDY QEAL RSLC QKRD GPRA TMKI MNYD EFQH CWSK FVYS QREL FEPW NNLP KYYI LLHI MLGE ILRH SMDP PTFT FNFN NEPW VRGR HETY LCYE VERM HNDT WVLL NQRR GFLC NQAP HKHG FLEG RHAE LCFL DVIP FWKL DLDQ DYRV TCFT SWSP CFSC AQEM AKFI SKNK HVSL CIFT ARIY DDQG RCQE GLRT LAE
Gene ID:	60489
Uniprot:	Q9HC16
Cellular Location:	Cytoplasm, Nucleus, P-body
Calculated MW:	9kDa/46kDa
Observed MW:

Synonyms:

APOBEC3G, A3G, ARCD, ARP-9, ARP9, CEM-15, CEM15, MDS019, bK150C2.7, dJ494G10.1

Background:

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene catalyzes site-specific deamination of both RNA and single-stranded DNA. The encoded protein has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.

UniProt Protein Function:	APOBEC3G: DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. May also prevent the transposition of a subset of retroelements. Binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing. Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; EC 3.5.4.-; Hydrolase; RNA processing Chromosomal Location of Human Ortholog: 22q13.1-q13.2 Cellular Component: apolipoprotein B mRNA editing enzyme complex; cytoplasm; cytosol; ribonucleoprotein complex Molecular Function:protein binding; protein homodimerization activity; deoxycytidine deaminase activity; zinc ion binding; RNA binding; cytidine deaminase activity Biological Process: negative regulation of retroviral genome replication; base conversion or substitution editing; viral reproduction; negative regulation of viral genome replication; innate immune response; cytidine deamination; positive regulation of defense response to virus by host; negative regulation of viral reproduction; defense response to virus
NCBI Summary:	This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene catalyzes site-specific deamination of both RNA and single-stranded DNA. The encoded protein has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity. [provided by RefSeq, Mar 2017]
UniProt Code:	Q9HC16
NCBI GenInfo Identifier:	44887683
NCBI Gene ID:	60489
NCBI Accession:	Q9HC16.1
UniProt Related Accession:	Q9HC16
Molecular Weight:
NCBI Full Name:	DNA dC->
NCBI Synonym Full Names:	apolipoprotein B mRNA editing enzyme catalytic subunit 3G
NCBI Official Symbol:	APOBEC3G
NCBI Official Synonym Symbols:	A3G; ARCD; ARP9; ARP-9; CEM15; CEM-15; MDS019; bK150C2.7; dJ494G10.1
NCBI Protein Information:	DNA dC->dU-editing enzyme APOBEC-3G
UniProt Protein Name:	DNA dC->dU-editing enzyme APOBEC-3G
UniProt Synonym Protein Names:	APOBEC-related cytidine deaminase; APOBEC-related protein; ARCD; APOBEC-related protein 9; ARP-9; CEM-15; CEM15; Deoxycytidine deaminase; A3G
UniProt Gene Name:	APOBEC3G
UniProt Entry Name:	ABC3G_HUMAN

	Western blot analysis of extracts of PC-3 cells, using APOBEC3G antibody (CAB13598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
	Immunohistochemistry of paraffin-embedded rat lung using APOBEC3G Rabbit pAb (CAB13598) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using APOBEC3G Rabbit pAb (CAB13598) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using APOBEC3G Rabbit pAb (CAB13598) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using APOBEC3G Rabbit pAb (CAB13598) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-APOBEC3G Antibody (CAB1459)

Anti-APOBEC3G Antibody (CAB17199)

APOBEC3G Antibody (PACO07791)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)