Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using APRR7 antibody (CAB22255) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The APRR7 Polyclonal Antibody (CAB22255) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting APRR7 in human samples, making it suitable for use in Western blot applications.The APRR7 protein, also known as APRR1, is a key component of the circadian clock system, playing a crucial role in maintaining proper physiological rhythms. Dysregulation of APRR7 has been linked to disruptions in sleep-wake cycles, mood disorders, and other circadian-related conditions. Therefore, studying the expression and function of APRR7 is essential for advancing our understanding of circadian biology and potentially developing targeted therapies for related disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Arabidopsis thaliana samples.
Product Name:
APRR7 Polyclonal Antibody
SKU:
CAB22255
Size:
20μL, 100μL
Reactivity:
Arabidopsis thaliana
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Seedling, Inflorescences, Before inflorescence, Rosette leaf
Observed MW:
80kDa
PRR7 and PRR9 are partially redundant essential components of a temperature-sensitive circadian system. CCA1 and LHY had a positive effect on PRR7 expression levels. Acts as transcriptional repressor of CCA1 and LHY.
Purification Method
Affinity purification
Gene ID
831793
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using APRR7 Rabbit pAb (CAB22255) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
