Anti-APRT Antibody (CAB5456)

Antibody Name:	Anti-APRT Antibody
Antibody SKU:	CAB5456
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT (NP_000476.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, 22Rv1, THP-1, A-549, K-562, HeLa, Mouse intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT (NP_000476.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADS ELQL VEQR IRSF PDFP TPGV VFRD ISPV LKDP ASFR AAIG LLAR HLKA THGG RIDY IAGL DSRG FLFG PSLA QELG LGCV LIRK RGKL PGPT LWAS YSLE YGKA ELEI QKDA LEPG QRVV VVDD LLAT GGTM NAAC ELLG RLQA EVLE CVSL VELT SLKG REKL APVP FFSL LQYE
Gene ID:	353
Uniprot:	P07741
Cellular Location:	Cytoplasm
Calculated MW:	14kDa/19kDa
Observed MW:	20kDa

Synonyms:

APRT, AMP, APRTD

Background:

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2, 8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	APRT: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD); also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones. Belongs to the purine/pyrimidine phosphoribosyltransferase family.
UniProt Protein Details:	Protein type:Nucleotide Metabolism - purine; Transferase; EC 2.4.2.7 Chromosomal Location of Human Ortholog: 16q24 Cellular Component: nucleoplasm; cytoplasm; cytosol Molecular Function:adenine binding; adenine phosphoribosyltransferase activity; AMP binding Biological Process: grooming behavior; lactation; cellular response to insulin stimulus; nucleobase, nucleoside and nucleotide metabolic process; adenine salvage; purine salvage; purine base metabolic process Disease: Adenine Phosphoribosyltransferase Deficiency
NCBI Summary:	Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P07741
NCBI GenInfo Identifier:	114074
NCBI Gene ID:	353
NCBI Accession:	P07741.2
UniProt Secondary Accession:	P07741,Q3KP55, Q68DF9, G5E9J2,
UniProt Related Accession:	P07741
Molecular Weight:	14,557 Da
NCBI Full Name:	Adenine phosphoribosyltransferase
NCBI Synonym Full Names:	adenine phosphoribosyltransferase
NCBI Official Symbol:	APRT
NCBI Official Synonym Symbols:	AMP; APRTD
NCBI Protein Information:	adenine phosphoribosyltransferase; AMP diphosphorylase; AMP pyrophosphorylase; transphosphoribosidase
UniProt Protein Name:	Adenine phosphoribosyltransferase
Protein Family:	Probable adenine phosphoribosyltransferase
UniProt Gene Name:	APRT
UniProt Entry Name:	APT_HUMAN

	Western blot analysis of extracts of various cell lines, using APRT antibody (CAB5456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of A549 cells using APRT antibody (CAB5456).

Antibodies

Anti-APRT Antibody (CAB13946)

APRT Antibody (PACO07800)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)