Anti-ARF5 Antibody (CAB3737)

Antibody Name:	Anti-ARF5 Antibody
Antibody SKU:	CAB3737
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF5 (NP_001653.1).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, A375, Mouse liver, Mouse brain, Mouse spleen, Mouse kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF5 (NP_001653.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGLT VSAL FSRI FGKK QMRI LMVG LDAA GKTT ILYK LKLG EIVT TIPT IGFN VETV EYKN ICFT VWDV GGQD KIRP LWRH YFQN TQGL IFVV DSND RERV QESA DELQ KMLQ EDEL RDAV LLVF ANKQ DMPN AMPV SELT DKLG LQHL RSRT WYVQ ATCA TQGT GLYD GLDW LSHE LSKR
Gene ID:	381
Uniprot:	P84085
Cellular Location:	Cytoplasm, Golgi apparatus, Lipid-anchor, Membrane, perinuclear region
Calculated MW:	20kDa
Observed MW:	16kDa

Synonyms:

ARF5

Background:

This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization.

UniProt Protein Function:	ARF5: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.
UniProt Protein Details:	Protein type:G protein, monomeric, ARF; G protein; G protein, monomeric; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 7q31.3 Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; plasma membrane Molecular Function:GTPase activity; protein binding; GTP binding Biological Process: vesicle-mediated transport; protein transport; metabolic process; small GTPase mediated signal transduction
NCBI Summary:	This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010]
UniProt Code:	P84085
NCBI GenInfo Identifier:	51316990
NCBI Gene ID:	381
NCBI Accession:	P84085.2
UniProt Secondary Accession:	P84085,P26437,
UniProt Related Accession:	P84085
Molecular Weight:	180
NCBI Full Name:	ADP-ribosylation factor 5
NCBI Synonym Full Names:	ADP-ribosylation factor 5
NCBI Official Symbol:	ARF5
NCBI Protein Information:	ADP-ribosylation factor 5
UniProt Protein Name:	ADP-ribosylation factor 5
Protein Family:	Auxin response factor
UniProt Gene Name:	ARF5
UniProt Entry Name:	ARF5_HUMAN

	Western blot analysis of extracts of various cell lines, using ARF5 antibody (CAB3737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of HeLa cells using ARF5 antibody (CAB3737) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-ARF5 Antibody (CAB19986)[KO Validated]

ARF5 Antibody (PACO07809)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)