The ARFGAP2 Antibody (CAB17791) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically targets the ARFGAP2 protein, allowing for detection and analysis in various cell types and tissues, making it an ideal choice for studies in cell biology and molecular biology.ARFGAP2 is a member of the ADP-ribosylation factor GTPase-activating protein (ARF-GAP) family, and it plays a crucial role in maintaining cellular homeostasis by modulating vesicle trafficking and membrane fusion events.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ARFGAP2 Antibody
SKU:
CAB17791
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IRZ, ZFP289, ZNF289, NBLA10535, ARFGAP2
Positive Sample:
THP-1, Mouse kidney, Mouse heart, Rat testis, Rat lung
Cellular Localization:
Cytosol, Golgi Apparatus, Plasma Membrane.
Calculated MW:
57kDa
Observed MW:
57kDa
Predicted to enable GTPase activator activity. Predicted to be involved in COPI coating of Golgi vesicle. Located in Golgi apparatus; cytosol; and plasma membrane.
Purification Method
Affinity purification
Gene ID
84364
RRID
AB_2768419
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from THP-1 cells, using ARFGAP2 Rabbit pAb (CAB17791) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Western blot analysis of various lysates using ARFGAP2 Rabbit pAb (CAB17791) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
