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Anti-ARHGAP25 Antibody (CAB1223)

SKU:
CAB1223
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 2 Anti-ARHGAP25 Antibody CAB1223
  • Cell Biology Antibodies 2 Anti-ARHGAP25 Antibody CAB1223
  • Cell Biology Antibodies 2 Anti-ARHGAP25 Antibody CAB1223
  • Cell Biology Antibodies 2 Anti-ARHGAP25 Antibody CAB1223
  • Cell Biology Antibodies 2 Anti-ARHGAP25 Antibody CAB1223
€139 - €419
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Description

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Antibody Name:Anti-ARHGAP25 Antibody
Antibody SKU:CAB1223
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human ARHGAP25 (NP_001159748.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:COS-7, OVCAR3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human ARHGAP25 (NP_001159748.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSLG QSAC LFLS IARS RSVM TGEQ MAAF HPSS TPNP LERP IKMG WLKK QRSI VKNW QQRY FVLR AQQL YYYK DEED TKPQ GCMY LPGC TIKE IATN PEEA GKFV FEII PASW DQNR MGQD SYVL MASS QAEM EEWV KFLR RVAG TPCG AVFG QRLD ETVA YEQK FGPH LVPI LVEK CAEF ILEH GRNE EGIF RLPG QDNL VKQL RDAF DAGE RPSF DRDT DVHT VASL LKLY LRDL PEPV VPWS QYEG FLLC GQLT NADE AKAQ QELM KQLS ILPR DNYS LLSY ICRF LHEI QLNC AVNK MSVD NLAT VIGV NLIR SKVE DPAV IMRG TPQI QRVM TMMI RDHE VLFP KSKD IPLS PPAQ KNDP KKAP VA
Gene ID:9938
Uniprot:P42331
Cellular Location:
Calculated MW:51kDa/69kDa/72kDa/73kDa
Observed MW:70kDa
Synonyms:ARHGAP25, HEL-S-308, KAIA0053
Background:
UniProt Protein Function:ARHGAP25: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: cytosol

Molecular Function:GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity

NCBI Summary:ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]
UniProt Code:P42331
NCBI GenInfo Identifier:238054314
NCBI Gene ID:9938
NCBI Accession:P42331.2
UniProt Secondary Accession:P42331,Q8IXQ2, A8K2Y1, B7Z498, E9PFQ7, G5E9G2,
UniProt Related Accession:P42331
Molecular Weight:645
NCBI Full Name:Rho GTPase-activating protein 25
NCBI Synonym Full Names:Rho GTPase activating protein 25
NCBI Official Symbol:ARHGAP25  
NCBI Official Synonym Symbols:KAIA0053; HEL-S-308  
NCBI Protein Information:rho GTPase-activating protein 25; epididymis secretory protein Li 308; rho-type GTPase-activating protein 25
UniProt Protein Name:Rho GTPase-activating protein 25
UniProt Synonym Protein Names:Rho-type GTPase-activating protein 25
UniProt Gene Name:ARHGAP25  
UniProt Entry Name:RHG25_HUMAN
Anti-ARHGAP25 Antibody (CAB1223)Western blot analysis of extracts of various cell lines, using ARHGAP25 antibody (CAB1223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-ARHGAP25 Antibody (CAB1223)Immunohistochemistry of paraffin-embedded human kidney using ARHGAP25 antibody (CAB1223) at dilution of 1:200 (40x lens).
Anti-ARHGAP25 Antibody (CAB1223)Immunohistochemistry of paraffin-embedded Human gastric using ARHGAP25 antibody (CAB1223) at dilution of 1:100 (40x lens).
Anti-ARHGAP25 Antibody (CAB1223)Immunofluorescence analysis of Jurkat cells using ARHGAP25 Polyclonal Antibody (CAB1223) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-ARHGAP25 Antibody (CAB1223)Immunofluorescence analysis of L929 cells using ARHGAP25 Polyclonal Antibody (CAB1223) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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