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Anti-ARHGAP5 Antibody (CAB3587)

SKU:
CAB3587
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 8 Anti-ARHGAP5 Antibody CAB3587
  • Cell Biology Antibodies 8 Anti-ARHGAP5 Antibody CAB3587
  • Cell Biology Antibodies 8 Anti-ARHGAP5 Antibody CAB3587
€139 - €419
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Description

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Antibody Name:Anti-ARHGAP5 Antibody
Antibody SKU:CAB3587
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human ARHGAP5 (NP_001164.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, 293T, HepG2, PC-12, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human ARHGAP5 (NP_001164.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EGLY RVSG NKTD QDNI QKQF DQDH NINL VSME VTVN AVAG ALKA FFAD LPDP LIPY SLHP ELLE AAKI PDKT ERLH ALKE IVKK FHPV NYDV FRYV ITHL NRVS QQHK INLM TADN LSIC FWPT LMRP DFEN REFL STTK IHQS VVET FIQQ CQFF FYNG EIVE TTNI VAPP PPSN PGQL VEPM VPLQ LPPP LQPQ LIQP QLQT DPLG II
Gene ID:394
Uniprot:Q13017
Cellular Location:Cytoplasm, Membrane, Peripheral membrane protein
Calculated MW:26kDa/27kDa/172kDa
Observed MW:185kDa
Synonyms:ARHGAP5, GFI2, RhoGAP5, p190-B, p190BRhoGAP
Background:Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:ARHGAP5: a GTPase activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of p120GAP. Negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP.
UniProt Protein Details:

Protein type:GAPs; Motility/polarity/chemotaxis; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function:GTPase activity; protein binding; GTP binding; SH2 domain binding

Biological Process: regulation of small GTPase mediated signal transduction; mammary gland development; metabolic process; small GTPase mediated signal transduction; positive regulation of mesenchymal cell proliferation; cell adhesion; regulation of cell size; Rho protein signal transduction; positive regulation of cell migration

NCBI Summary:Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q13017
NCBI GenInfo Identifier:190358871
NCBI Gene ID:394
NCBI Accession:Q13017.2
UniProt Related Accession:Q13017
Molecular Weight:
NCBI Full Name:Rho GTPase-activating protein 5
NCBI Synonym Full Names:Rho GTPase activating protein 5
NCBI Official Symbol:ARHGAP5  
NCBI Official Synonym Symbols:GFI2; p190-B; RhoGAP5; p190BRhoGAP  
NCBI Protein Information:rho GTPase-activating protein 5
UniProt Protein Name:Rho GTPase-activating protein 5
UniProt Synonym Protein Names:Rho-type GTPase-activating protein 5; p190-B
UniProt Gene Name:ARHGAP5  
UniProt Entry Name:RHG05_HUMAN
Anti-ARHGAP5 Antibody (CAB3587)Western blot analysis of extracts of various cell lines, using ARHGAP5 antibody (CAB3587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-ARHGAP5 Antibody (CAB3587)Immunohistochemistry of paraffin-embedded rat brain using ARHGAP5 antibody (CAB3587) at dilution of 1:100 (40x lens).
Anti-ARHGAP5 Antibody (CAB3587)Immunofluorescence analysis of HeLa cells using ARHGAP5 antibody (CAB3587) at dilution of 1:100. Blue: DAPI for nuclear staining.

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