The RhoGAP Antibody (CAB3587) is a high-quality antibody developed for reliable detection and analysis of target proteins. Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. RRID AB_2765168 Gene ID 394 Swiss Prot Synonym GFI2; p190-B; RhoGAP5; p190BRhoGAP; RhoGAP
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RhoGAP Antibody
SKU:
CAB3587
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GFI2, p190-B, RhoGAP5, p190BRhoGAP, RhoGAP
Positive Sample:
HeLa, Jurkat, 293T, HepG2, PC-12, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Membrane, Peripheral Membrane Protein.
Calculated MW:
172kDa
Observed MW:
185kDa
Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. RRID AB_2765168 Gene ID 394 Swiss Prot Synonym GFI2; p190-B; RhoGAP5; p190BRhoGAP; RhoGAP
Purification Method:
Affinity purification
Gene ID:
394
RRID:
AB_2765168
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RhoGAP Rabbit pAb (CAB3587) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using RhoGAP Rabbit pAb (CAB3587) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
