Anti-ASCL4 Antibody (CAB14439)

Antibody Name:	Anti-ASCL4 Antibody
Antibody SKU:	CAB14439
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human ASCL4 (NP_982260.2).

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human ASCL4 (NP_982260.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MMET RKPA ERLA LPYS LRTA PLGV PGTL PGLP RRDP LRVA LRLD AACW EWAR SGCA RGWQ YLPV PLDS AFEP AFLR KRNE RERQ RVRC VNEG YARL RDHL PREL ADKR LSKV ETLR AAID YIKH LQEL LERQ AWGL EGAA GAVP QRRA ECNS DGES KASS APSP SSEP EEGG
Gene ID:	121549
Uniprot:	Q6XD76
Cellular Location:	Nucleus
Calculated MW:	19kDa
Observed MW:	19kDa

Synonyms:	ASCL4, HASH4, bHLHa44
Background:

UniProt Protein Function:	ASCL4: Could be a transcriptional regulator involved in skin development.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 12q23.3 Cellular Component: nucleus Molecular Function:protein dimerization activity; DNA binding Biological Process: regulation of transcription from RNA polymerase II promoter; skin development; transcription, DNA-dependent
NCBI Summary:	Basic helix-loop-helix transcription factors, such as ASCL4, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q6XD76
NCBI GenInfo Identifier:	206597481
NCBI Gene ID:	121549
NCBI Accession:	NP_982260.2
UniProt Secondary Accession:	Q6XD76,Q7RTS2,
UniProt Related Accession:	Q6XD76
Molecular Weight:	19,253 Da
NCBI Full Name:	achaete-scute homolog 4
NCBI Synonym Full Names:	achaete-scute family bHLH transcription factor 4
NCBI Official Symbol:	ASCL4
NCBI Official Synonym Symbols:	HASH4; bHLHa44
NCBI Protein Information:	achaete-scute homolog 4; ASH-4; class II bHLH protein ASCL4; achaete-scute complex-like 4; achaete-scute-like protein 4; achaete-scute complex homolog 4; class A basic helix-loop-helix protein 44
UniProt Protein Name:	Achaete-scute homolog 4
UniProt Synonym Protein Names:	Achaete-scute-like protein 4; Class A basic helix-loop-helix protein 44; bHLHa44
Protein Family:	Achaete-scute
UniProt Gene Name:	ASCL4
UniProt Entry Name:	ASCL4_HUMAN

	Western blot analysis of extracts of various cell lines, using ASCL4 antibody (CAB14439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human lung cancer using ASCL4 antibody (CAB14439) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using ASCL4 antibody (CAB14439) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using ASCL4 antibody (CAB14439) at dilution of 1:100 (40x lens).

Secondary Antibody