Aspartate Assay Kit (BA0078)
Aspartate Assay Kit - InformationAssay Genie's Aspartate Assay Kit provides a simple, direct and automation-ready procedure for measuring aspartate concentration. Aspartate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the aspartate concentration in the sample.
ApplicationsFor quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism.
Aspartate Assay Kit - Key Features
- Sensitive and accurate. Linear detection range: 2 to 400 aspartate for colorimetric assays and 1 to 50 for fluorimetric assays.
Aspartate Assay Kit - Data Sheet
|Kit Includes||Developer: 10 mL AST Enzyme: 240 mL Dye Reagent: 120 mL Cosubstrate: 600 mL ODC Enzyme: 120 mL Aspartate Standard: 400 mL|
|Kit Requires||Pipetting devices, clear or black flat-bottom 96-well plates, plate reader or centrifuge tubes.|
|Method of Detection||OD570nm, or FL530/585nm|
|Detection Limit||OD, FL: 2, 1|
|Samples||Plasma, serum, tissue, culture media etc|
|Protocol Length||60 min|
|Storage||Store all kit components at -20 °C.|
|Shelf Life||6 months|
More DetailsASPARTATE , a nonessential amino acid, is a precursor to several other amino acids and is an excitatory neurotransmitter. Aspartate is involved in the urea cycle and gluconeogenesis.